CNTF Receptor alpha anticorps (Extracellular)

N° du produit ABIN7884884

Cet anticorps anti-CNTF Receptor alpha Polyclonal Lapin (ABIN7884884) détecte spécifiquement CNTF Receptor alpha dans WB, IHC, IF, FACS et LCI. L’anticorps est réactif avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour CNTF Receptor alpha anticorps (Extracellular) (ABIN7884884)

Antigène: CNTF Receptor alpha (CNTFR) (Ciliary Neurotrophic Factor Receptor (CNTFR))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CNTF Receptor alpha est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Classe de qualité: Carrier-free

Détail du produit anti-CNTF Receptor alpha anticorps

Épitope: AA 164-178, Extracellular

Fonction: A Rabbit Polyclonal Antibody to CNTF Receptor α

Homologie: Mouse,human - identical

Purification: Affinity purified on immobilized antigen.

Immunogène: CHIRYMHLFSTIKYK, corresponding to amino acid residues 164-178 of rat CNTFRalpha

Isotype: IgG

Information d'application

Indications d'application:

WB: 1:200

FC: 5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:400

IP: The optimal concentration should be determined by the user

Commentaires:

Negative Control: (ABIN7235141)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Agent conservateur: Without preservative

Stock: -20 °C

Stockage commentaire: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Détails sur CNTF Receptor alpha

Antigène: CNTF Receptor alpha (CNTFR) (Ciliary Neurotrophic Factor Receptor (CNTFR))

Autre désignation: Ciliary neurotrophic factor receptor subunit alpha

Sujet:

Synonyms: Ciliary neurotrophic factor receptor subunit alpha, CNTF receptor alpha, CNTFR alpha

Description: CNTF is a polypeptide trophic factor, member of the alpha-helical cytokine superfamily1. It was initially purified from the chick eye on the basis of its ability to promote survival of E8 chick ciliary ganglion neurons in culture2. CNTF is synthesized by glia both in the CNS and PNS3 and it has been demonstrated that CNTF is ubiquitously distributed in neurons and glia throughout the rodent brain4. CNTF effects are mediated by a tripartite receptor complex consisting of two signal-transducing subunits (leukemia inhibitory factor receptor, gp130) and a CNTF-specific ligand-binding-subunit (CNTFRα)5.CNTFRα is anchored to the membrane by a glycosyl-phosphatidylinositol (GPI) linkage and lacks the intracellular signaling moiety. It can be cleaved from the GPI anchor to yield a soluble form of the receptor (sCNTFRα)6,7.CNTF binds to CNTFRα, the latter associates with gp130 which recruits LIFR. This leads to the activation of a signaling cascade involving the JAK/STAT pathway7.CNTFRα knockout mice die within 24 hours after birth as opposed to CNTF knock animals which are viable. This strongly suggest that CNTFRα has other ligands besides CNTF. Indeed, CLC/CLF complex (cardiotrophin like cytokine/cytokine-like factor-1) binds CNTFRα and activates a similar signaling pathway to that of CNTF8.

ID gène: 313173

UniProt: Q08406

Pathways: Signalistation JAK/STAT, Feeding Behaviour