Nerve Growth Factor anticorps

N° du produit ABIN7885156

L’anticorps anti-Nerve Growth Factor Polyclonal Lapin est utilisé pour la détection de Nerve Growth Factor dans des échantillons de Humain, Rat et Souris. Il a été validé pour WB, IHC et IF.

Aperçu rapide pour Nerve Growth Factor anticorps (ABIN7885156)

Antigène: Nerve Growth Factor (NGF)

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Nerve Growth Factor est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Classe de qualité: Carrier-free

Détail du produit anti-Nerve Growth Factor anticorps

Fonction: A Rabbit Polyclonal Antibody to Nerve Growth Factor

Homologie: Rat- 115,120 amino acid residues identical, human- 107

Purification: Affinity purified on immobilized antigen.

Immunogène: Highly purified 2.5 S mouse NGF 

Isotype: IgG

Information d'application

Indications d'application:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Commentaires:

Negative Control: (ABIN7235998)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Agent conservateur: Without preservative

Stock: -20 °C

Stockage commentaire: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Détails sur Nerve Growth Factor

Antigène: Nerve Growth Factor (NGF)

Autre désignation: Nerve growth factor

Sujet:

Synonyms: Nerve growth factor

Description: The neurotrophins ("neuro" means nerve and "trophe" means nutrient) are a family of soluble, basic growth factors which regulate neuronal development, maintenance, survival and death in the CNS and the PNS.1 NGF, the first member of the family to be discovered, was originally purified as a factor able to support survival of sympathetic and sensory spinal neurons in culture.2 It is synthesized and secreted by sympathetic and sensory target organs and provides trophic support to neurons as they reach their final target.3 Neurotrophin secretion also increases in the nervous system following injury. Schwann cells, fibroblasts, and activated mast cells normally synthesize NGF constitutively, however direct trauma and induced cytokines combine to increase neurotrophin production in these cells after injury.4NGF is purified in three forms: the 7S, 2.5S and β. The 7S, 130 kDa, form occurs naturally in mouse submaxillary glands, and is a multimeric protein composed of two α, one β and two γ subunits. The name is derived from its sedimentation co-efficient, 7S. The biologically active subunit is the β, which is a 26 kDa dimer composed of two identical 120 amino acid chains held together by hydrophobic interactions.5 The 2.5S form is 9 amino acids shorter than the β form, because of proteolysis that occurs during the purification process.6 The structural hallmark of all the neurotrophins is the characteristic arrangement of the disulfide bridges known as the cysteine knot, which has been found in other growth factors such as PDGF.7 There is a 95.8 % homology between the rat and mouse forms, and a 85 % homology between the human and mouse.NGF has been shown to regulate neuronal survival, development function and plasticity.8 Recently, involvement of NGF in processes not involving neuronal cells has been shown, such as asthma,9 psoriasis10 and wound healing.11The biological effects of NGF are mediated by two receptors: TrkA, which is specific for NGF, and p75, which binds all the neurotrophins.12

ID gène: 18049

UniProt: P01139

Pathways: Regulation of Cell Size