Synaptophysin anticorps (Intravesicular Loop)

N° du produit ABIN7885555

L’anticorps anti-Synaptophysin Polyclonal Lapin est utilisé pour la détection de Synaptophysin dans des échantillons de Rat et Souris. Il a été validé pour WB, IHC et IF.

Aperçu rapide pour Synaptophysin anticorps (Intravesicular Loop) (ABIN7885555)

Antigène: Synaptophysin (SYP)

Reactivité: Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Synaptophysin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Classe de qualité: Carrier-free

Détail du produit anti-Synaptophysin anticorps

Épitope: AA 178-190, Intravesicular Loop

Fonction: A Rabbit Polyclonal Antibody to Synaptophysin

Homologie: Rat,mouse,human - 12,13 amino acid residues identical

Purification: Affinity purified on immobilized antigen.

Immunogène: CRQTGNT(S)KELRD, corresponding to amino acid residues 178-190 of rat synaptophysin

Isotype: IgG

Information d'application

Indications d'application:

WB: 1:400

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:100

IP: The optimal concentration should be determined by the user

Commentaires:

Negative Control: (ABIN7237323)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Agent conservateur: Without preservative

Stock: -20 °C

Stockage commentaire: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Détails sur Synaptophysin

Antigène: Synaptophysin (SYP)

Autre désignation: Major synaptic vesicle protein p38

Sujet:

Synonyms: Major synaptic vesicle protein p38, SYP

Description: Synaptophysin is the major integral membrane protein of small synaptic vesicles1-3. It belongs to a family of proteins which includes synaptogyrin and synaptoporin. The protein contains 4 transmembrane domains and cytoplasmic N- and C-termini4. There are two intravesicular loops that contain disulfide bonds. The protein is N-glycosylated on the first intravesicular loop, a modification important for the synaptic vesicle targeting of synaptophysin. Indeed, a mutant form of the protein, unable to undergo glycolysation, leads to the accumulation of the protein in the cell body of neurons indicating that N-glycosylation is required for the synaptic localization of synaptophysin5. The C-terminal tail is extensively phosphorylated on Tyr residues. However, the biological significance of the post-translational modification is not yet determined6.Although exact and specific role of synaptophysin remains a mystery, a study suggests that the protein is required for efficient endocytosis of synaptic vesicles in cultured hippocampal neurons6.Since synaptophysin is exclusively detected in synaptic vesicles, it is widely used as a marker for presynaptic vesicles.Genetic screening in humans and behavioral studies in rodents show that loss of synaptophysin expression or expression of a truncated form of the protein may be attributed to mental retardation and/or learning deficits7,8. Interestingly though, synaptic transmission in synaptophysin knockout mice remains normal6.

ID gène: 24804

UniProt: P07825

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of long-term Neuronal Synaptic Plasticity