RAPTOR anticorps (pSer792)

N° du produit ABIN745478

L’anticorps Lapin Polyclonal anti-RAPTOR a été validé pour ELISA, IF (cc), IF (p), IHC (p) et IHC (fro). Il convient pour détecter RAPTOR dans des échantillons de Humain, Souris et Poisson zèbre (Danio rerio).

Aperçu rapide pour RAPTOR anticorps (pSer792) (ABIN745478)

Antigène: RAPTOR

Reactivité: Humain, Souris, Poisson zèbre (Danio rerio)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RAPTOR est non-conjugé

Application: ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-RAPTOR anticorps

Épitope: pSer792

Réactivité croisée: Humain, Souris, Poisson zèbre (Danio rerio)

Homologie: Rat,Dog,Cow,Sheep,Pig,Horse,Chicken,Guinea Pig

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic phosphopeptide derived from mouse Raptor around the phosphorylation site of Ser792

Isotype: IgG

Information d'application

Indications d'application: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Détails sur RAPTOR

Antigène: RAPTOR

Autre désignation: Raptor

Sujet:

Synonyms: ARaptor phospho S792, Raptor phospho Ser792, p-Raptor Ser792, p150 target of rapamycin TOR scaffold protein containing WD repeats, P150 target of rapamycin TOR-scaffold protein, Regulatory Associated Protein of mTOR, KIAA1303, RPTOR_HUMAN, Regulatory-associated protein of mTOR, p150 target of rapamycin TOR-scaffold protein.

Background: mTOR controls cell growth, in part by regulating p70 S6 kinase alpha (p70alpha) and eukaryotic initiation factor 4E binding protein 1 (4EBP1). Raptor is a 150 kDa mTOR binding protein that also binds 4EBP1 and p70alpha. The binding of Raptor to mTOR is necessary for the mTOR-catalyzed phosphorylation of 4EBP1 in vitro, and it strongly enhances the mTOR kinase activity toward p70alpha. Rapamycin or amino acid withdrawal increases, whereas insulin strongly inhibits, the recovery of 4EBP1 and raptor on 7-methyl-GTP Sepharose. Partial inhibition of raptor expression by RNA interference (RNAi) reduces mTOR-catalyzed 4EBP1 phosphorylation in vitro. RNAi of C. elegans raptor yields an array of phenotypes that closely resemble those produced by inactivation of Ce-TOR. Thus, raptor is an essential scaffold for the mTOR-catalyzed phosphorylation of 4EBP1 and mediates TOR action in vivo.

Pathways: L'effet Warburg