BRD2 anticorps (AA 58-232)

N° du produit ABIN7938062

L’anticorps anti-BRD2 Polyclonal Lapin est utilisé pour la détection de BRD2 dans des échantillons de Humain. Il a été validé pour ChIP-seq, ChIP et CUT&RUN.

Aperçu rapide pour BRD2 anticorps (AA 58-232) (ABIN7938062)

Antigène: BRD2 (Bromodomain Containing 2 (BRD2))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp BRD2 est non-conjugé

Application: ChIP DNA-Sequencing (ChIP-seq), Chromatin Immunoprecipitation (ChIP), Cleavage Under Targets and Release Using Nuclease (CUT&RUN)

Détail du produit anti-BRD2 anticorps

Épitope: AA 58-232

Fonction: BRD2 antibody (pAb)

Purification: Affinity Purified

Immunogène: This BRD2 antibody was raised against a recombinant protein comprising amino acids 58-232 of human BRD2 (NP_005095 ).

Isotype: IgG

Information d'application

Indications d'application: ChIP-Seq: 6 µL per ChIP CUT&RUN: 1 µL per 50 µL reaction

Restrictions: For Research Use only

Stockage

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Détails sur BRD2

Antigène: BRD2 (Bromodomain Containing 2 (BRD2))

Autre désignation: BRD2

Sujet:

Background_Plain: BRD2 (Bromodomain-containing protein 2) belongs to the BET subclass of proteins, which are characterized by two N-terminal bromodomains and one ET (Extra Terminal) domain. BRDs associate with chromatin through their bromodomains that recognize acetylated histone lysine residues. Bromodomains function as 'readers' of these epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the acetylated nucleosomal targets. The ET domain functions as a protein binding motif and exerts atypical serine-kinase activity. The BET family consists of at least four members in mouse and human, BRD2 (also referred to as FSRG1, RING3), BRD3 (FSRG2, ORFX), BRD4 (FSRG4, MCAP/HUNK1), and BRDT (FSRG3, BRD6). BRD proteins are related to the female Sterile Homeotic protein in Drosophila, a gene required maternally for proper expression of other homeotic genes, such as Ubx, which is involved in pattern formation. BRD2 causes elevated protein kinase activity in leukemias. Transgenic mice overexpressing BRD2 in the lymphoid system develop diffuse large-cell lymphoma. BRD2 has been shown to interact with E2F1 and with histone H4 acetylated at Lys12 via its two bromodomains. BRD2 may play a role in spermatogenesis or folliculogenesis. Genetic evidence links the BRD2 gene to both juvenile myoclonic epilepsy and photoparoxysomal responses.

Aliases: Bromodomain Containing 2, O27.1.1, RING3

Poids moléculaire: 110 kDa

NCBI Accession: NP_005095

Pathways: Chromatin Binding, SARS-CoV-2 Protein Interactome, Phosphorylation & l'infection par le SRAS-CoV-2