ULK1 anticorps (pSer556) (HRP)

N° du produit ABIN746732

L’anticorps Lapin Polyclonal anti-ULK1 a été validé pour WB, ELISA, IHC (p) et IHC (fro). Il convient pour détecter ULK1 dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour ULK1 anticorps (pSer556) (HRP) (ABIN746732)

Antigène: ULK1 (Unc-51 Like Kinase 1 (ULK1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ULK1 est conjugé à/à la HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-ULK1 anticorps (HRP)

Épitope: pSer556

Réactivité croisée: Humain, Souris, Rat

Homologie: Pig,Horse

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic phosphopeptide derived from human ATG1 around the phosphorylation site of Ser556

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Conseil sur la manipulation: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détails sur ULK1

Antigène: ULK1 (Unc-51 Like Kinase 1 (ULK1))

Autre désignation: ATG1

Sujet:

Synonyms: ATG1, ATG1A, UNC51, hATG1, Unc51.1, Serine/threonine-protein kinase ULK1, Autophagy-related protein 1 homolog, Unc-51-like kinase 1, ULK1, KIAA0722

Background: Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR, however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation.

ID gène: 8408

UniProt: O75385

Pathways: Regulation of Cell Size, Autophagy