WEE1 anticorps (pSer642)

N° du produit ABIN746873

L’anticorps Lapin Polyclonal anti-WEE1 a été validé pour WB, ELISA, IF (cc), IF (p), IHC (p) et IHC (fro). Il convient pour détecter WEE1 dans des échantillons de Humain.

Aperçu rapide pour WEE1 anticorps (pSer642) (ABIN746873)

Antigène: WEE1 (WEE1 Homolog (S. Pombe) (WEE1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp WEE1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-WEE1 anticorps

Épitope: pSer642

Homologie: Human,Mouse,Rat,Dog,Cow,Pig,Rabbit

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic phosphopeptide derived from human Wee1 around the phosphorylation site of Ser642 [SV(p-S)LT]

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Détails sur WEE1

Antigène: WEE1 (WEE1 Homolog (S. Pombe) (WEE1))

Autre désignation: Wee1

Sujet:

Synonyms: WEE1A, WEE1hu, Wee1-like protein kinase, Wee1A kinase, WEE1

Background: Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.

ID gène: 7465

UniProt: P30291

Pathways: Cycle Cellulaire, Mitotic G1-G1/S Phases, M Phase