APEX1 anticorps (AA 2-318) (HRP)

N° du produit ABIN7969571

Cet anticorps anti-APEX1 Monoclonal Souris conjugué à HRP (Clone 5C11) (ABIN7969571) détecte spécifiquement APEX1 dans WB, ELISA et IHC. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour APEX1 anticorps (AA 2-318) (HRP) (ABIN7969571)

Antigène: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp APEX1 est conjugé à/à la HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone: 5C11

Détail du produit anti-APEX1 anticorps (HRP)

Épitope: AA 2-318

Fonction: Anti-APE1 APEX1 Antibody (monoclonal, 5C11) HRP Conjugated

Specificité: No cross reactivity with other proteins.

Réactivité croisée (Details): No cross-reactivity with other proteins.

Homologie: Human APE1 shares 94%,93% amino acid (aa) sequence identity with mouse,rat APE1,respectively.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human APE1 recombinant protein (Position: P2-L318). Human APE1 shares 94% and 93% amino acid (aa) sequence identity with mouse and rat APE1, respectively.

Isotype: IgG2b

Information d'application

Indications d'application: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4.

Stock: -20 °C

Stockage commentaire: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur APEX1

Antigène: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Autre désignation: APEX1

Sujet:

Background: APEX1, also called apurinic endonuclease (APE), is a DNA repair enzyme having apurinic/apyrimidinic (AP) endonuclease, 3-prime, 5-prime-exonuclease, DNA 3-prime repair diesterase, and DNA 3-prime-phosphatase activities. The human APEX1 gene consists of 5 exons spanning 2.64 kb and exists as a single copy in the haploid genome. Using in situ hybridization, the APEX1 gene is mapped to 14q11.2-q12. The predicted APEX1 protein, which contained probable nuclear transport signals, was identified as a member of a family of DNA repair enzymes found in lower organisms. The abundance of the large form of APEX1 was increased in leiomyoma extracts relative to myometrial tissue extracts, and the large form was dominant in cell lines derived from leiomyosarcomas. The exonuclease activity of nuclear APEX1 can remove the anti-HIV nucleoside analogs AZT and D4T from the 3-prime terminus of a nick more efficiently than can cytosolic exonucleases.

Gene Full Name: apurinic/apyrimidinic endodeoxyribonuclease 1

ID gène: 328

UniProt: P27695

Pathways: Réparation de l'ADN, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus