DDB2 anticorps (AA 201-300)

N° du produit ABIN747803

L’anticorps Lapin Polyclonal anti-DDB2 a été validé pour WB, ELISA, IF (cc), IF (p), IHC (fro) et IHC (p). Il convient pour détecter DDB2 dans des échantillons de Souris et Rat.

Aperçu rapide pour DDB2 anticorps (AA 201-300) (ABIN747803)

Antigène: DDB2 (Damage-Specific DNA Binding Protein 2, 48kDa (DDB2))

Reactivité: Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DDB2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-DDB2 anticorps

Épitope: AA 201-300

Specificité: This antibody may have a minor secondary cross-reactivity to TNFAIP3-interacting protein 2 due to a 63 % non-sequential sequence similarity in the immunogen region.

Réactivité croisée: Souris, Rat

Homologie: Human,Cow,Horse,Rabbit

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human DDB2

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Détails sur DDB2

Antigène: DDB2 (Damage-Specific DNA Binding Protein 2, 48kDa (DDB2))

Autre désignation: DDB2

Sujet:

Synonyms: DDBB, UV-DDB2, DNA damage-binding protein 2, DDB p48 subunit, Damage-specific DNA-binding protein 2, UV-damaged DNA-binding protein 2, UV-DDB 2, DDB2

Background: Required for DNA repair. Binds to DDB1 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as the substrate recognition module for the DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex DDB1-CUL4-ROC1 (also known as CUL4-DDB-ROC1 and CUL4-DDB-RBX1). The DDB1-CUL4-ROC1 complex may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. The DDB1-CUL4-ROC1 complex also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. Isoform D1 and isoform D2 inhibit UV-damaged DNA repair.

ID gène: 1643

UniProt: Q92466

Pathways: Réparation de l'ADN