TdT anticorps (AA 316-509) (Fluoro550)

N° du produit ABIN7984879

L’anticorps anti-TdT Polyclonal Lapin est utilisé pour la détection de TdT dans des échantillons de Humain. Il a été validé pour FACS.

Aperçu rapide pour TdT anticorps (AA 316-509) (Fluoro550) (ABIN7984879)

Antigène: TdT (DNTT) (Deoxynucleotidyltransferase, terminal (DNTT))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TdT est conjugé à/à la Fluoro550

Application: Flow Cytometry (FACS)

Détail du produit anti-TdT anticorps (Fluoro550)

Épitope: AA 316-509

Fonction: Anti-TdT/DNTT Antibody Fluoro550 Conjugated

Specificité: No cross reactivity with other proteins.

Réactivité croisée (Details): No cross-reactivity with other proteins

Homologie: Human TdT shares 81% amino acid (aa) sequence identity with mouse TdT.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human TdT recombinant protein (Position: K316-A509). Human TdT shares 81% amino acid (aa) sequence identity with mouse TdT.

Isotype: IgG

Information d'application

Indications d'application: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Date de péremption: 12 months

Détails sur TdT

Antigène: TdT (DNTT) (Deoxynucleotidyltransferase, terminal (DNTT))

Autre désignation: DNTT

Sujet:

Background: Terminal Deoxynucleotidyl Transferase, also known as TDT and terminal transferase, is a unique DNA polymerase without template ion catalyzes the addition of deoxyribonucleotides onto the 3-prime-hydroxyl end of DNA primers. Its gene is mapped to the region 10q23-q24. And TDT cDNA contains an open reading frame of 1,530 basepairs corresponding to a protein containing 510 amino acids. TDT may be responsible for inserting nucleotides (N regions) at the V (H)-D and D-J (H) junctions of immunoglobulin genes. The enzyme is present in immature thymocytes, some bone marrow cells, transformed pre-B and pre-T cell lines, and leukemia cells. Additionally, TDT catalyses the addition of nucleotides to the 3' terminus of a DNA molecule. Unlike most DNA polymerases it does not require a template. The preferred substrate of this enzyme is a 3'-overhang, but it can also add nucleotides to blunt or recessed 3' ends. Cobalt is a necessary cofactor.

Gene Full Name: DNA nucleotidylexotransferase

Sequence Similarities: Belongs to the DNA polymerase type-X family.

ID gène: 1791

UniProt: P04053

Pathways: Réparation de l'ADN