EPAS1 anticorps (Middle Region) (HRP)

N° du produit ABIN7987703

Cet anticorps anti-EPAS1 Polyclonal Lapin conjugué à HRP (ABIN7987703) détecte spécifiquement EPAS1 dans WB, IHC et ELISA. L’anticorps est réactif avec des échantillons de Humain, Rat et Souris.

Aperçu rapide pour EPAS1 anticorps (Middle Region) (HRP) (ABIN7987703)

Antigène: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp EPAS1 est conjugé à/à la HRP

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Détail du produit anti-EPAS1 anticorps (HRP)

Épitope: Middle Region

Fonction: Anti-HIF-2-alpha/EPAS1 Antibody HRP Conjugated

Specificité: No cross reactivity with other proteins.

Réactivité croisée (Details): No cross-reactivity with other proteins

Homologie: identical to the related mouse,rat sequence.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence in the middle region of human HIF-2-alpha, identical to the related mouse and rat sequence.

Isotype: IgG

Information d'application

Indications d'application: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4.

Stock: -20 °C

Stockage commentaire: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur EPAS1

Antigène: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Autre désignation: EPAS1

Sujet:

Background: HIF-2 alpha is also designated EPAS1 whose gene is mapped to 2p21-p16. The predicted mouse protein is 88 % identical to human EPAS1. The human EPAS1 gene contains 15 exons and spans at least 120 kb. The positions of the introns within the genomic region encoding the N-terminal bHLH-PAS domains of EPAS1 and AHR are similar, suggesting that the 5-prime ends of the 2 genes may have arisen from a gene duplication event1. Moreover, the predicted protein shares 48 % sequence identity with HIF1-alpha, a bHLH-PAS transcription factor that induces EPO gene expression in cultured cells in response to hypoxia. Like HIF1A, EPAS1 binds to and activates transcription from the HIF1A response element derived from the 3-prime flanking region of the EPO gene. EPAS1 is predominantly expressed in highly vascularized tissues of adult humans and in endothelial cells of the mouse adult and embryo. Furthermore, EPAS1 may represent an important regulator of vascularization, perhaps involving the regulation of endothelial cell gene expression in response to hypoxia2. HIF2A is expressed at relatively higher levels in villus sections of placenta and in lung samples compared with other tissues examined3. In addition, The variation in EPAS1 influences the relative contribution of aerobic and anaerobic metabolism and hence the maximum sustainable metabolic power for a given event duration4.

Gene Full Name: endothelial PAS domain protein 1

Sequence Similarities: Contains 1 bHLH (basic helix-loop-helix) domain.

ID gène: 29452

UniProt: Q9JHS1

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2, L'effet Warburg