IRF3 anticorps (AA 1-419)

N° du produit ABIN8001197

Cet anticorps Souris Monoclonal détecte spécifiquement IRF3 dans WB, IF, FACS et ICC. Il présente une réactivité avec des échantillons de Souris et Rat.

Aperçu rapide pour IRF3 anticorps (AA 1-419) (ABIN8001197)

Antigène: IRF3 (Interferon Regulatory Factor 3 (IRF3))

Reactivité: Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp IRF3 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Classe de qualité: Carrier-free

Clone: 3B4

Détail du produit anti-IRF3 anticorps

Épitope: AA 1-419

Fonction: Anti-IRF3 Antibody (monoclonal, 3B4)

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-IRF3 Antibody.(monoclonal, 3B4)-3. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived mouse IRF3 recombinant protein (Position: M1-I419).

Isotype: IgG2a

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Mouse Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détails sur IRF3

Antigène: IRF3 (Interferon Regulatory Factor 3 (IRF3))

Autre désignation: Irf3

Sujet:

Background: IRF3 (interferon regulatory factor 3) is a member of the interferon regulatory transcription factor (IRF) family. The IRF3 gene is mapped on 19q13.33. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. IRF3 plays an important role in the innate immune system's response to viral infection. Aggregated MAVS have been found to activate IRF3 dimerization. Although IRF3 increased transcriptional activity from an ISRE-containing promoter, expression of IRF3 as a Gal4 fusion protein did not activate expression of a chloramphenicol acetyltransferase (CAT) < a href=https://www.bosterbio.com/cells/reporter-cell-lines style=color:#ea8d28>reporter gene containing repeats of the Gal4-binding sites. Translocation of IRF3 was accompanied by an increase in serine and threonine phosphorylation. The transcriptional activators CREBBP and EP300 coimmunoprecipitated with IRF3 only subsequent to viral infection, and the authors stated that these are also subunits of DRAF1.

Gene Full Name: interferon regulatory factor 3

Poids moléculaire: 50-55 kDa

ID gène: 54131

UniProt: P70671

Pathways: Signalisation TLR, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades