MBD4 anticorps (C-Term) (PE)

N° du produit ABIN8009625

L’anticorps anti-MBD4 Polyclonal Lapin est utilisé pour la détection de MBD4 dans des échantillons de Humain, Souris et Rat. Il a été validé pour FACS.

Aperçu rapide pour MBD4 anticorps (C-Term) (PE) (ABIN8009625)

Antigène: MBD4 (Methyl-CpG Binding Domain Protein 4 (MBD4))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MBD4 est conjugé à/à la PE

Application: Flow Cytometry (FACS)

Détail du produit anti-MBD4 anticorps (PE)

Épitope: C-Term

Fonction: Anti-MBD4 Antibody PE Conjugated

Specificité: No cross reactivity with other proteins.

Réactivité croisée (Details): No cross-reactivity with other proteins

Homologie: identical to the related rat,mouse sequences.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human MBD4, identical to the related rat and mouse sequences.

Isotype: IgG

Information d'application

Indications d'application: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Date de péremption: 12 months

Détails sur MBD4

Antigène: MBD4 (Methyl-CpG Binding Domain Protein 4 (MBD4))

Autre désignation: MBD4

Sujet:

Background: MBD4 (Methyl-CpG-Binding Domain Protein 4), also known as MED1, is a protein that in humans is encoded by the MBD4 gene. MBD4 specifically binds methylated DNA, colocalizes with methylated sequences, and is likely to mediate the effects of DNA methylation in mammalian cells (Hendrich and Bird, 1998). Riccio et al. (1999) mapped the MBD4 gene to chromosome 3q21-q22 by FISH. Hendrich and Bird (1998) found that both MBD2 and MBD4 specifically bound methylated DNA in vitro and colocalized with methylated sequences in vivo. They concluded that MBD2 and MBD4 are likely to be mediators of the effects of DNA methylation in mammalian cells. Hendrich et al. (1999) showed that MBD4 contains a methyl-CpG-binding domain that can efficiently remove thymine or uracil from mismatched CpG sites in vitro. Furthermore, the methyl-CpG-binding domain of MBD4 binds preferentially to 5-methylcytosine CpG-TpG mismatches--the primary product of deamination at methyl-CpG.

Gene Full Name: methyl-CpG binding domain 4, DNA glycosylase

Sequence Similarities: Contains 1 MBD (methyl-CpG-binding) domain.

ID gène: 8930

UniProt: O95243

Pathways: Réparation de l'ADN