Mitochondrially Encoded NADH Dehydrogenase 4 (MT-ND4) (AA 10-459) anticorps (HRP)

N° du produit ABIN8010801

L’anticorps anti-Mitochondrially Encoded NADH Dehydrogenase 4 Polyclonal Lapin est utilisé pour la détection de Mitochondrially Encoded NADH Dehydrogenase 4 dans des échantillons de Humain. Il a été validé pour WB, ELISA et IHC.

Aperçu rapide pour Mitochondrially Encoded NADH Dehydrogenase 4 (MT-ND4) (AA 10-459) anticorps (HRP) (ABIN8010801)

Antigène: Mitochondrially Encoded NADH Dehydrogenase 4 (MT-ND4)

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Détail du produit

Épitope: AA 10-459

Fonction: Anti-MT-ND4 Antibody HRP Conjugated

Specificité: No cross reactivity with other proteins.

Réactivité croisée (Details): No cross-reactivity with other proteins.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human MT-ND4 recombinant protein (Position: M10-S459).

Isotype: IgG

Information d'application

Indications d'application: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4.

Stock: -20 °C

Stockage commentaire: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: Mitochondrially Encoded NADH Dehydrogenase 4 (MT-ND4)

Autre désignation: MT-ND4

Sujet:

Background: NADH-ubiquinone oxidoreductase chain 4 is a protein that in humans is encoded by the mitochondrial gene MT-ND4. MT-ND4 is a subunit of the respiratory chain Complex I that is believed to belong to the minimal assembly of core proteins required to catalyze NADH dehydrogenation and electron transfer to ubiquinone (coenzyme Q10). Initially, NADH binds to Complex I and transfers two electrons to the isoalloxazine ring of the flavin mononucleotide (FMN) prosthetic arm to form FMNH2. The electrons are transferred through a series of iron-sulfur (Fe-S) clusters in the prosthetic arm and finally to coenzyme Q10 (CoQ), which is reduced to ubiquinol (CoQH2). The flow of electrons changes the redox state of the protein, resulting in a conformational change and pK shift of the ionizable side chain, which pumps four hydrogen ions out of the mitochondrial matrix.

Gene Full Name: NADH dehydrogenase subunit 4

ID gène: 4538

UniProt: P03905