Prohibitin 2 anticorps (AA 1-299)

N° du produit ABIN8025683

Cet anticorps Souris Monoclonal détecte spécifiquement Prohibitin 2 dans WB, IHC et FACS. Il présente une réactivité avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour Prohibitin 2 anticorps (AA 1-299) (ABIN8025683)

Antigène: Prohibitin 2 (PHB2)

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Prohibitin 2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Classe de qualité: Carrier-free

Clone: 2B5

Détail du produit anti-Prohibitin 2 anticorps

Épitope: AA 1-299

Fonction: Anti-REA/PHB2 Antibody (monoclonal, 2B5)

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-REA/PHB2 Antibody.(monoclonal, 2B5). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human REA/PHB2 recombinant protein (Position: M1-K299).

Isotype: IgG2b

Information d'application

Indications d'application: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détails sur Prohibitin 2

Antigène: Prohibitin 2 (PHB2)

Autre désignation: PHB2

Sujet:

Background: PHB2 (Prohibitin 2), also called Repressor of Estrogen Receptor Activity (REA), is a protein that in humans is encoded by the PHB2 gene. The International Radiation Hybrid Mapping Consortium mapped the PHB2 gene to chromosome 12. Montano et al. (1999) showed that REA enhanced the potency of a dominant-negative ER-alpha mutant and antiestrogens as suppressors of ER-alpha activity in Chinese hamster ovary cells. When coexpressed with wildtype ER-alpha or ER-beta (ESR2), REA suppressed activation of a < a href=https://www.bosterbio.com/cells/reporter-cell-lines style=color:#ea8d28>reporter gene in a dose-dependent manner. REA had no effect on reporter activity in the absence of liganded ER, and it had no effect on the transcriptional activities of other hormone receptors. Mutation analysis showed that an N-terminal domain and a central domain of REA were required for its repressor activity.

Gene Full Name: prohibitin 2

Poids moléculaire: 32 kDa

ID gène: 11331

UniProt: Q99623

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling