Topoisomerase II alpha anticorps (C-Term) (HRP)

N° du produit ABIN8047562

Cet anticorps Lapin Polyclonal détecte spécifiquement Topoisomerase II alpha dans WB, IHC et ELISA. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour Topoisomerase II alpha anticorps (C-Term) (HRP) (ABIN8047562)

Antigène: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Topoisomerase II alpha est conjugé à/à la HRP

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Détail du produit anti-Topoisomerase II alpha anticorps (HRP)

Épitope: C-Term

Fonction: Anti-Topoisomerase II alpha/TOP2A Antibody HRP Conjugated

Specificité: No cross reactivity with other proteins.

Réactivité croisée (Details): No cross-reactivity with other proteins

Homologie: different from the related rat sequence by three amino acids,and different from the related mouse sequence by four amino acids.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human Topoisomerase II alpha, different from the related rat sequence by three amino acids, and different from the related mouse sequence by four amino acids.

Isotype: IgG

Information d'application

Indications d'application: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4.

Stock: -20 °C

Stockage commentaire: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur Topoisomerase II alpha

Antigène: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Autre désignation: TOP2A

Sujet:

Background: The human topoisomerase II enzyme is encoded by a single-copy gene which is mapped to 17q21-q22. The TOP2A gene spans approximately 30 kb and contains 35 exons. Furthermore, DNA topoisomerases are enzymes that control and alter the topologic states of DNA in both prokaryotes and eukaryotes. Topoisomerase II from eukaryotic cells catalyzes the relaxation of supercoiled DNA molecules, catenation, decatenation, knotting, and unknotting of circular DNA. It appears likely that the reaction catalyzed by topoisomerase II involves the crossing-over of 2 DNA segments. There are about 100,000 Molecules of topoisomerase II per HeLa cell nucleus, constituting about 0.1 % of the nuclear extract. DNA topoisomerase II-alpha is associated with the pol II holoenzyme and is a required component of chromatin-dependent coactivation. Specific inhibitors of topoisomerase II blocked transcription on chromatin templates, but did not affect transcription on naked templates. Addition of purified topoisomerase II-alpha reconstituted chromatin-dependent activation activity in reactions with core pol II.

Gene Full Name: DNA topoisomerase II alpha

Sequence Similarities: Belongs to the type II topoisomerase family.

ID gène: 7153

UniProt: P11388

Pathways: Cycle Cellulaire, Mitotic G1-G1/S Phases