KAP1 anticorps (AA 699-835)

N° du produit ABIN8049790

Cet anticorps anti-KAP1 Monoclonal Souris (Clone 9E3) (ABIN8049790) détecte spécifiquement KAP1 dans WB, IHC, IF, FACS, ICC et IHC (fro). L’anticorps est réactif avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour KAP1 anticorps (AA 699-835) (ABIN8049790)

Antigène: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp KAP1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Classe de qualité: Carrier-free

Clone: 9E3

Détail du produit anti-KAP1 anticorps

Épitope: AA 699-835

Fonction: Anti-KAP1/TRIM28 Antibody (monoclonal, 9E3)

Specificité: No cross reactivity with other proteins.

Réactivité croisée (Details): No cross-reactivity with other proteins.

Homologie: Human KAP1 shares 94.9% amino acid (aa) sequence identity with both mouse,rat KAP1.

Attributs du produit: Anti-KAP1/TRIM28 Antibody.(monoclonal, 9E3)-2. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human KAP1 recombinant protein (Position: A699-P835). Human KAP1 shares 94.9% amino acid (aa) sequence identity with both mouse and rat KAP1.

Isotype: IgG2a

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human, Rat Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human Immunofluorescence, 2 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détails sur KAP1

Antigène: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Autre désignation: TRIM28

Sujet:

Background: Tripartite motif-containing 28 (TRIM28), also known as transcriptional intermediary factor 1β (TIF1β) and KAP1 (KRAB-associated protein-1), is a protein that in humans is encoded by the TRIM28 gene. The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. KAP1 is a ubiquitously expressed protein involved in many critical functions including: transcriptional regulation, cellular differentiation and proliferation, DNA damage repair, viral suppression, and apoptosis. Its functionality is dependent upon post-translational modifications. Phosphorylation of KAP1 acts as a deactivator of the protein in many of its mechanisms while sumoylation acts as an activator.

Gene Full Name: tripartite motif containing 28

Poids moléculaire: 100 kDa

ID gène: 10155

UniProt: Q13263

Pathways: Signalisation Hedgehog, Positive Regulation of Response to DNA Damage Stimulus