MMP1 anticorps (Cleaved-Ile271)

N° du produit ABIN8122794

L’anticorps anti-MMP1 Polyclonal Lapin est utilisé pour la détection de MMP1 dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB, IHC et ELISA.

Aperçu rapide pour MMP1 anticorps (Cleaved-Ile271) (ABIN8122794)

Antigène: MMP1 (Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MMP1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Détail du produit anti-MMP1 anticorps

Épitope: Cleaved-Ile271

Fonction: MMP1 (Cleaved-Ile271) Antibody

Specificité: This antibody detects endogenous levels of Human MMP1 (Cleaved-Ile271, protein was cleaved amino acid sequence between 270-271 )

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Immunogène: Synthesized peptide derived from human MMP1 (Cleaved-Ile271)

Isotype: IgG

Information d'application

Indications d'application: WB 1:500-2000,IHC 1:50-300, ELISA 2000-20000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20°C/1 year

Date de péremption: 12 months

Détails sur MMP1

Antigène: MMP1 (Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1))

Autre désignation: MMP1

Sujet:

Synonyms: Interstitial collagenase (EC 3.4.24.7, Fibroblast collagenase, Matrix metalloproteinase-1, MMP-1) [Cleaved into: 22 kDa interstitial collagenase, 27 kDa interstitial collagenase]

Background: catalytic activity:Cleavage of the triple helix of collagen at about three-quarters of the length of the molecule from the N-terminus, at 775-Gly-|-Ile-776 in the alpha-1(I) chain. Cleaves synthetic substrates and alpha-macroglobulins at bonds where P1' is a hydrophobic residue.,cofactor:Binds 2 zinc ions per subunit.,cofactor:Binds 4 calcium ions per subunit.,domain:The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.,domain:There are two distinct domains in this protein, the catalytic N-terminal, and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases).,enzyme regulation:Can be activated without removal of the activation peptide.,function:Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.,online information:Collagenase entry,PTM:Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.,similarity:Belongs to the peptidase M10A family.,similarity:Contains 4 hemopexin-like domains.,subunit:Interacts with HIV-1 Tat.,

Gene Name: MMP1 CLG

Protein Name: MMP1 (Cleaved-Ile271)

ID gène: 4312

UniProt: P03956