POLA1 anticorps (AA 1231-1350)

N° du produit ABIN763391

L’anticorps Lapin Polyclonal anti-POLA1 a été validé pour WB, ELISA, IHC (fro), IF (cc), IF (p) et IHC (p). Il convient pour détecter POLA1 dans des échantillons de Humain.

Aperçu rapide pour POLA1 anticorps (AA 1231-1350) (ABIN763391)

Antigène: POLA1 (DNA Polymerase alpha (POLA1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp POLA1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-POLA1 anticorps

Épitope: AA 1231-1350

Homologie: Human,Mouse,Rat,Dog,Horse,Rabbit

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human DPOLA/DNA polymerase alpha

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Détails sur POLA1

Antigène: POLA1 (DNA Polymerase alpha (POLA1))

Autre désignation: Dna Polymerase alpha

Sujet:

Synonyms: DNA polymerase alpha 1 catalytic subunit, DNA polymerase alpha catalytic subunit, DNA polymerase alpha catalytic subunit p180, DNA polymerase alpha p180 subunit, DPOLA_HUMAN, P180, p180 subunit, POLA, POLA1, Polymerase DNA directed, alpha 1, catalytic subunit, Polymerase DNA-directed, alpha 70kD.

Background: Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes.

ID gène: 5422

Pathways: SARS-CoV-2 Protein Interactome