Angiopoietin 1 anticorps (Internal Region)

N° du produit ABIN768552

Détail du produit anti-Angiopoietin 1 anticorps

Antigène: Angiopoietin 1 (ANGPT1)

Épitope: Internal Region

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Angiopoietin 1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Fonction: ANGPT1

Séquence: PDFSSQKLQH

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Porc, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Classe de qualité: Verified

Immunogène: Peptide with sequence C-PDFSSQKLQH, from the internal region of the protein sequence according to NP_001137.2.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: In transfected HEK293 transiently expressing full-length Human ANGPT1 (myc and DYKDDDDK tagged), a band of approx. 48 kDa was observed. No bands were observed in mock-transfected HEK293 and the 48 kDa plus a 75 kDa band was observed using anti

Peptide ELISA: antibody detection limit dilution 1:32000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the plasma membranes of A431 and HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentr

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur Angiopoietin 1

Antigène: Angiopoietin 1 (ANGPT1)

Autre désignation: ANGPT1 (ANGPT1 Produits)

Sujet: ANGPT1, angiopoietin 1, AGP1, AGPT, ANG1

Poids moléculaire: 48kDa

ID gène: 284, 11600, 89807

NCBI Accession: NP_001137

Pathways: Signalisation RTK, Glycosaminoglycan Metabolic Process