POU2AF1 anticorps (C-Term)

N° du produit ABIN768553

Cet anticorps Chèvre Polyclonal détecte spécifiquement POU2AF1 dans WB, IHC et ELISA. Il présente une réactivité envers Humain.

Aperçu rapide pour POU2AF1 anticorps (C-Term) (ABIN768553)

Antigène: POU2AF1 (POU Class 2 Associating Factor 1 (POU2AF1))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp POU2AF1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Classe de qualité: Verified

Détail du produit anti-POU2AF1 anticorps

Épitope: C-Term

Fonction: POU2AF1

Séquence: DSDAYALNHT LSVE

Réactivité croisée: Boeuf (Vache), Chien, Humain

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-DSDAYALNHTLSVE, from the C Terminus of the protein sequence according to NP_006226.2.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: In paraffin embedded Human Spleen shows nuclear staining in the germinal center with stronger nucleoli staining in some of the B cells. Recommended concentration, 3-5 μg/mL.

Western Blot: In transfected HEK293 transiently expressing full-length Human POU2AF1 (myc and DYKDDDDK tagged), a band of approx. 38 kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag

Peptide ELISA: antibody detection limit dilution 1:8000.

Commentaires:

Purchase the +ve control! The transfected lysate used to QC this product is available for sale. Please email sales@everestbiotech.com to request datasheet, pricing and delivery information.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur POU2AF1

Antigène: POU2AF1 (POU Class 2 Associating Factor 1 (POU2AF1))

Autre désignation: POU2AF1

Sujet: POU2AF1, POU class 2 associating factor 1, BOB1, OBF-1, OBF1, OCAB, POU domain, class 2, associating factor 1

Poids moléculaire: 38kDa

ID gène: 5450

NCBI Accession: NP_006226