Albumin anticorps (HRP)
Aperçu rapide pour Albumin anticorps (HRP) (ABIN784429)
Antigène
Voir toutes Albumin (ALB) AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Specificité
- Tested in immunoelectrophoresis and double radial immunodiffusion against pooled normal rat serum and purified rat albumin. One characteristic precipitin line is obtained against pooled normal rat serum using different antigen/antibody concentration ratio's. Precipitin lines against normal rat serum and purified rat albumin give a reaction of full identity. Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to mammalian serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentrations of the reactants and the sensitivity of the assay arrangement. This antiserum fraction has been tested for cross-reactivity by double radial immunodiffusion against several species sera with the following results: ++ mouse, guinea pig + monkey ± horse, human, swine - rabbit, sheep, goat, canine, chicken, bovine
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Attributs du produit
- Molar Ratio: 1,7
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Purification
- DEAE-column Chromatography
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Immunogène
- Albumin is a stable small polypeptide with a strong antigenicity. Its molecular weight is about 69,000. It has a high mobility in electrophoresis, shows macro-heterogeneity especially under pathological conditions and it can bind a large number of physiological and non-physiological molecules. Albumin is isolated from rat serum by sequential precipitation and purified by ion exchange chromatography and affinity chromatography. Freund’s complete adjuvant is used in the first step of the immunization procedure.
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Isotype
- IgG
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Indications d'application
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Reconstitution
- Restore with 1 mL sterile distilled water
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Concentration
- 10 mg/mL
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Buffer
- PBS, pH 7.2 No preservative added, as it may interfere with the antibody activity. No foreign proteins added.
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Conseil sur la manipulation
- Avoid repeated freezing and thawing.
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Stock
- 4 °C/-20 °C
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Stockage commentaire
- Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one week or (in aliquots) at -20 °C for longer.
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- Albumin (ALB)
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Autre désignation
- Albumin
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Sujet
- Synonyms: ALB, BSA, HSA, Serum Albumin
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ID gène
- 24186
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NCBI Accession
- NP_599153
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Pathways
- Lipid Metabolism
Antigène
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