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LPO anticorps (Biotin)

Cet anticorps anti-LPO est un anticorps Lapin Polyclonal détectant LPO dans WB, IP, EIA, DB, ID, IF et RIA. Adapté pour Boeuf (Vache).
N° du produit ABIN784497

Aperçu rapide pour LPO anticorps (Biotin) (ABIN784497)

Antigène

Voir toutes LPO Anticorps
LPO (Lactoperoxidase (LPO))

Reactivité

  • 11
  • 8
  • 6
  • 3
  • 2
  • 2
  • 1
Boeuf (Vache)

Hôte

  • 15
  • 4
  • 4
Lapin

Clonalité

  • 19
  • 4
Polyclonal

Conjugué

  • 15
  • 6
  • 2
Cet anticorp LPO est conjugé à/à la Biotin

Application

  • 20
  • 14
  • 13
  • 10
  • 7
  • 2
  • 2
  • 1
Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Dot Blot (DB), Immunodiffusion (ID), Immunofluorescence (IF), Radioimmunoassay (RIA)
  • Specificité

    The reactivity of the antiserum is restricted to lactoperoxidase as tested in immunoelectrophoresis and radial immunodiffusion. Using various antiserum concentrations against fresh bovine milk a single precipitin line is obtained. No precipitation reaction is obtained with normal bovine serum or plasma. Cross-reactivity: The antiserum does not cross-react with any other component of bovine serum. Interspecies cross-reactivity is a normal feature of antibodies, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail, but reactivity with lactoperoxidase from goat and sheep may be expected.

    Purification

    DEAE-column Chromatography

    Immunogène

    Lactoperoxidase (EC 1.11.1.7) is one of the major enzymes in bovine milk. The enzyme has a molecular weight of 80,000. It is isolated from pooled bovine milk. Freund’s complete adjuvant is used in the first step of the immunization procedure.

    Isotype

    IgG
  • Indications d'application

    Optimal working dilution should be determined by the investigator.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Reconstitution

    Restore by adding 1 mL sterile distilled water

    Concentration

    10 mg/mL

    Buffer

    PBS, pH 7.2 without preservatives and foreign proteins.

    Agent conservateur

    Without preservative

    Conseil sur la manipulation

    Avoid repeated freezing and thawing.

    Stock

    4 °C/-20 °C

    Stockage commentaire

    Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one week or (in aliquots) at -20 °C for longer.
  • Antigène

    LPO (Lactoperoxidase (LPO))

    Autre désignation

    Lactoperoxidase

    Sujet

    Lactoperoxidase is a natural antibacterial agent in bovine milk, a medium otherwise rich in its ability to support bacterial growth. Milk lactoperoxidase levels vary among species, ranging from little or none in humans to very high concentrations in guinea pigs. Interestingly, lactoperoxidase from milk is identical to the lacrimal and salivary forms of the enzyme. In addition, the enzyme catalyzes the hydrogen peroxide oxidation of iodine and can be employed in labelling proteins with radioiodine.Synonyms: EC=1.11.1.7, LPO, Lactoperoxidase, SAPX, SPO, Salivary peroxidase

    ID gène

    280844

    NCBI Accession

    NP_776358
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