AQP2 anticorps (pSer269)

N° du produit ABIN784597

Cet anticorps Lapin Polyclonal détecte spécifiquement AQP2 dans WB et IHC (fro). Il présente une réactivité envers Rat, Souris, Boeuf (Vache) et Chien et a été mentionné dans 1 publication.

Aperçu rapide pour AQP2 anticorps (pSer269) (ABIN784597)

Antigène: AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))

Reactivité: Rat, Souris, Boeuf (Vache), Chien

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp AQP2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-AQP2 anticorps

Épitope: pSer269

Specificité: Specific for ~29k AQP2 protein phosphorylated at Ser269. This antibody also recognizes the glycosylated form of AQP2 at ~37k. Immunolabeling of the AQP2 band is blocked by preadsorption with the phospho-peptide used as antigen but not by the corresponding dephospho-peptide.

Réactivité croisée (Details): Species reactivity (expected):Bovine, Canine, Mouse.
Species reactivity (tested):Rat.

Purification: Affinity Chromatography.

Immunogène: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser269 of Rat Aquaporin 2.

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody undiluted (in aliquots) at -20 °C.

Références pour anticorps anti-AQP2 (ABIN784597)

Bogum, Faust, Zühlke, Eichhorst, Moutty, Furkert, Eldahshan, Neuenschwander, von Kries, Wiesner, Trimpert, Deen, Valenti, Rosenthal, Klussmann: "Small-molecule screening identifies modulators of aquaporin-2 trafficking." dans: Journal of the American Society of Nephrology : JASN, Vol. 24, Issue 5, pp. 744-58, (2013) (PubMed).

Détails sur AQP2

Antigène: AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))

Autre désignation: Aquaporin-2

Sujet: Aquaporin 2 (AQP2) is a hormonally regulated water channel located in the renal collecting duct. Mutations in the AQP2 gene cause hereditary nephrogenic diabetes insipidus in humans (Iolascon et al.,2007). A vasopressin induced cAMP increase results in the phosphorylation of AQP2 at serine-256 and its translocation from the intracellular vesicles to the apical membrane of principal cells (van Balkom et al., 2002). Recently, Serine-269 has been identified as a vasopressin-mediated phosphorylation site on AQP2 and as such has shown to potentiate plasma membrane retention of AQP2 (Hoffert JD et al., 2008).Synonyms: ADH water channel, AQP-CD, AQP2, Aquaporin-CD, Collecting duct water channel protein, WCH-CD, Water channel protein for renal collecting duct

ID gène: 25386

NCBI Accession: NP_037041

Pathways: Response to Water Deprivation