LIN28B anticorps (AA 242-246)

N° du produit ABIN871727

L’anticorps anti-LIN28B Polyclonal Lapin est utilisé pour la détection de LIN28B dans des échantillons de Humain. Il a été validé pour WB.

Aperçu rapide pour LIN28B anticorps (AA 242-246) (ABIN871727)

Antigène: LIN28B (Lin-28 Homolog B (LIN28B))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LIN28B est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-LIN28B anticorps

Épitope: AA 242-246

Specificité: The antibody detects endogenous levels of total LIN28B protein.

Purification: Affinity purified

Immunogène: Peptide sequence around AA 242-246 ( P-S-V-Q-K) derived from Human LIN28B Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

Information d'application

Indications d'application: Western blotting: 1:500-1:1000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Détails sur LIN28B

Antigène: LIN28B (Lin-28 Homolog B (LIN28B))

Autre désignation: LIN28B

Sujet: Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.

Poids moléculaire: 32 kDa

ID gène: 389

NCBI Accession: NP_001004

UniProt: Q6ZN17