KAP1 anticorps (AA 827-831)

N° du produit ABIN871735

Cet anticorps Lapin Polyclonal détecte spécifiquement KAP1 dans WB. Il présente une réactivité envers Humain.

Aperçu rapide pour KAP1 anticorps (AA 827-831) (ABIN871735)

Antigène: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp KAP1 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-KAP1 anticorps

Épitope: AA 827-831

Specificité: The antibody detects endogenous level of total TIF1b protein.

Purification: Affinity purified

Immunogène: Peptide sequence around AA 827-831 (L-S-G-G-P) derived from Human TIF1b. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

Information d'application

Indications d'application: Western blotting: 1:500-1:1000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Détails sur KAP1

Antigène: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Autre désignation: TIF1beta

Sujet: Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger.

Poids moléculaire: 110 kDa

ID gène: 10155

NCBI Accession: NP_005753

UniProt: Q13263

Pathways: Signalisation Hedgehog, Positive Regulation of Response to DNA Damage Stimulus