Western blotting 1:5000. Immunofluorescence 1:1000. Immunoprecipitation 1:100. ELISA 1:2000
Restrictions
For Research Use only
Format
Liquid
Concentration
1.0 mg/mL
Agent conservateur
Sodium azide
Précaution d'utilisation
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Stock
-20 °C
Antigène
DYKDDDDK Tag
Autre désignation
DYKDDDDK-Tag
Classe de substances
Tag
Sujet
This monoclonal antibody is produced by immunizing animals with a synthetic DYKDDDDK peptide (KLH-coupled). Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size they are unlikely to affect the tagged protein's biochemical properties. The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion.