DNASE1 anticorps (AA 101-200) (HRP)

N° du produit ABIN894181

Détail du produit anti-DNASE1 anticorps (HRP)

Antigène: DNASE1 (Deoxyribonuclease I (DNASE1))

Épitope: AA 101-200

Reactivité: Humain, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DNASE1 est conjugé à/à la HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Réactivité croisée: Humain, Rat

Homologie: Mouse,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human DNase I

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Conseil sur la manipulation: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détails sur DNASE1

Antigène: DNASE1 (Deoxyribonuclease I (DNASE1))

Autre désignation: DNase 1 (DNASE1 Produits)

Synonymes: anticorps LOC397802, anticorps DNL1, anticorps DRNI, anticorps AI788650, anticorps DNaseI, anticorps Dnl1, anticorps dnase1-A, anticorps zgc:92440, anticorps DNase I, anticorps deoxyribonuclease 1, anticorps deoxyribonuclease I, anticorps deoxyribonuclease I L homeolog, anticorps Deoxyribonuclease I, anticorps endA, anticorps LOC397802, anticorps DNASE1, anticorps Dnase1, anticorps dnase1.L, anticorps dnase1, anticorps CpipJ_CPIJ011184, anticorps CpipJ_CPIJ011185, anticorps CpipJ_CPIJ011186, anticorps CpipJ_CPIJ011187, anticorps CpipJ_CPIJ011190, anticorps CpipJ_CPIJ017803, anticorps Desal_1409, anticorps MPET_RS05255, anticorps METEV_RS02275, anticorps Plabr_0631

Sujet:

Synonyms: RNASE1, Dornase alfa, Deoxyribonuclease 1, Deoxyribonuclease I, Deoxyribonuclease1, DeoxyribonucleaseI, DNASE 1, DNase I lysosomal, DNASE1, DNaseI, DNL 1, DNL1, DRNI, Human urine deoxyribonuclease I, DNAS1_HUMAN.

Background: Deoxyribonuclease I gene is approximately 3.2 kb long with 9 exons separated by 8 introns. In the form of a bovine pancreatic enzyme preparation, it occupies an important place in the history of protein chemistry and enzymology: it was the first enzyme to be recognized as specific for DNA, it was the first DNase to be crystallized, and it was the first DNase for which a specific protein inhibitor was characterized. DNase I is a Ca2+ and Mg2+ dependant endonuclease. DNase I is synthesized in the pancreas and stored in zymogen granules. It has been used to reduce the viscosity of cystic fibrosis sputum. A DNase I-like enzyme appears to catalyze the degradation of chromatin to oligo- and mononucleosomes during apoptosis. A recent study has demonstrated an endonuclease with activity and antigenicity indistinguishable from DNase I in thymocytes, cells susceptible to apoptosis. DNase I is an endonuclease that hydrolyzes double-stranded or single stranded DNA preferentially at sites adjacent to pyrimidine nucleotides. The product of hydrolysis is a complex mixture of 5'-phosphate mononucleotides and oligonucleotides. In the presence of Mg ion, DNase I attacks each strand of DNA independently and the cleavage sites are random.

ID gène: 1773