Cytokeratin 1 anticorps (AA 141-240) (AbBy Fluor® 555)

N° du produit ABIN909638

L’anticorps Lapin Polyclonal anti-Cytokeratin 1 a été validé pour WB, FACS, IF (p) et IF (cc). Il convient pour détecter Cytokeratin 1 dans des échantillons de Humain, Rat, Souris et Boeuf (Vache). Il y a 1 publication disponible.

Aperçu rapide pour Cytokeratin 1 anticorps (AA 141-240) (AbBy Fluor® 555) (ABIN909638)

Antigène: Cytokeratin 1 (KRT1) (Keratin 1 (KRT1))

Reactivité: Humain, Rat, Souris, Boeuf (Vache)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Cytokeratin 1 est conjugé à/à la AbBy Fluor® 555

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Détail du produit anti-Cytokeratin 1 anticorps (AbBy Fluor® 555)

Épitope: AA 141-240

Réactivité croisée: Boeuf (Vache), Humain, Souris, Rat

Homologie: Dog,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human pan cytokeratin

Isotype: IgG

Information d'application

Indications d'application: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-Cytokeratin 1 (AbBy Fluor® 555) (ABIN909638)

Poruk, Blackford, Weiss, Cameron, He, Goggins, Rasheed, Wolfgang, Wood: "Circulating Tumor Cells Expressing Markers of Tumor-Initiating Cells Predict Poor Survival and Cancer Recurrence in Patients with Pancreatic Ductal Adenocarcinoma." dans: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 23, Issue 11, pp. 2681-2690, (2018) (PubMed).

Détails sur Cytokeratin 1

Antigène: Cytokeratin 1 (KRT1) (Keratin 1 (KRT1))

Autre désignation: Cytokeratin

Sujet:

Synonyms: pan-cytokeratin, pan-CK, pan CK, P-CK, wide spectrum Cytokeratin, Cytokeratins, [cytokeratins 1, 2,4,5,6,7,8,71,72,75,78].

Background: Cytokeratins are proteins of keratin-containing intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue. The cytokeratins are encoded by a family encompassing 30 genes. Among them, 20 are epithelial genes and the remaining 10 are specific for trichocytes. In the cytoplasm, the keratin filaments conform a complex network which extends from the surface of the nucleus to the cell membrane. Numerous accessory proteins are involved in the genesis and maintenance of such structure. This association between the plasma membrane and the nuclear surface provides important implications for the organization of the cytoplasm and cellular communication mechanisms. Apart from the relatively static functions provided in terms of supporting the nucleus and providing tensile strength to the cell, the cytokeratin networks undergo rapid phosphate exchanges mediated depolymerization, with important implications in the more dynamic cellular processes such as mitosis and post-mitotic period, cell movement and differentiation. Cytokeratins interact with desmosomes and hemidesmosomes, thus collaborating to cell-cell adhesion and basal cell-underlying connective tissue connection.