Ubiquitin anticorps (AA 1-76) (AbBy Fluor® 647)

N° du produit ABIN919177

Cet anticorps Lapin Polyclonal détecte spécifiquement Ubiquitin dans WB, FACS, IF (cc) et IF (p). Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour Ubiquitin anticorps (AA 1-76) (AbBy Fluor® 647) (ABIN919177)

Antigène: Ubiquitin (Ub)

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Ubiquitin est conjugé à/à la AbBy Fluor® 647

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Détail du produit anti-Ubiquitin anticorps (AbBy Fluor® 647)

Épitope: AA 1-76

Réactivité croisée: Humain, Souris, Rat

Homologie: Pig

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human Ubiquitin

Isotype: IgG

Information d'application

Indications d'application: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détails sur Ubiquitin

Antigène: Ubiquitin (Ub)

Autre désignation: Ubiquitin

Sujet:

Synonyms: HMG2, Polyubiquitin-C, UBC

Background: Ubiquitin: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair, Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation, Lys-29-linked is involved in lysosomal degradation, Lys-33-linked is involved in kinase modification, Lys-48-linked is involved in protein degradation via the proteasome, Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.

ID gène: 7316

UniProt: P0CG48

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway