His Tag anticorps

N° du produit ABIN920801

L’anticorps Poulet Polyclonal anti-His Tag a été validé pour WB et ELISA. Il convient pour détecter His Tag dans des échantillons de .

Aperçu rapide pour His Tag anticorps (ABIN920801)

Antigène: His Tag

Reactivité: Veuillez nous consulter SVP

Hôte: Poulet

Clonalité: Polyclonal

Conjugué: Cet anticorp His Tag est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-His Tag anticorps

Séquence: HHHHHH

Purification: Affinity purified

Information d'application

Indications d'application: Western blot: Use at 1:1,000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS pH 7.2 and 0.1% sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Détails sur His Tag

Antigène: His Tag

Autre désignation: Hexahistidine-Tag

Classe de substances: Tag

Sujet: Chickens were immunized with HHHHHH peptide conjugated to KLH. After multiple immunizations in Freund's adjuvant, eggs were collected and the immunoglobulin fraction prepared. Antibodies were immunoaffinity- purified using the peptide immobilized on a solid support. Specification: Antibody specificity was verified by ELISA against peptide conjugated to BSA (HHHHHH- BSA). A 1:8,000 dilution of the antibody gave an OD = 1.0 in a 15 minute reaction using HRP- conjugated goat anti-chicken IgY (IgG) at 1:10,000 at TMB as the substrate. Appropriate specificity controls were run.