50 mM HEPES pH 7.0, 0.1M Sodium Chloride with 0.2% BSA and 0.01% thimerosal.
Agent conservateur
Thimerosal (Merthiolate)
Précaution d'utilisation
This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C
Antigène
His Tag
Autre désignation
Hexahistidine-Tag
Classe de substances
Tag
Sujet
Chickens were immunized with HHHHHH peptide conjugated to KLH. After multiple immunizations in Freund's adjuvant, eggs were collected and the immunoglobulin fraction prepared. Antibodies were immunoaffinity- purified using the peptide immobilized on a solid support and the antibodies were conjugated with horseradish peroxidase (HRP). Specification: ELISA: HRP-conjugated chicken anti- hexahistidine diluted 1:10,000 gave an OD = 1.0 in a 15 minute reaction against peptide conjugated to a different carrier than used for anti-peptide purification.