WB: 1:1000-3000 Optimal conditions should be determined by the investigator.
Restrictions
For Research Use only
Format
Liquid
Concentration
Lot specific
Buffer
Supplied as a liquid in 10 mM PBS, pH 7.2, with 0.05 % NaN3
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.
Stock
4 °C/-20 °C
Stockage commentaire
Store at 4 °C for several days to weeks. Add 0.1 % NaN3 and store at -20 °C for longer periods.
Antigène
DYKDDDDK Tag
Autre désignation
DYKDDDD-Tag
Classe de substances
Tag
Sujet
DYKDDDDK tag is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. A DYKDDDDK-Tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a DYKDDDDK-Tag to this protein allows one to follow the protein with an antibody against the DYKDDDDK sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis. Synonyms: Monoclonal DYKDDDDK antibody, Anti DYKDDDDK antibody, DYKDDDDK antibody, DYKDDDDK antibody, FG4R antibody, DYKDDDDK tag antibody.