CD147 anticorps

N° du produit ABIN93979

Cet anticorps Souris Monoclonal détecte spécifiquement CD147 dans FACS et IHC (p). Il présente une réactivité envers Humain et Porc et a été mentionné dans 2+ publications.

Aperçu rapide pour CD147 anticorps (ABIN93979)

Antigène: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Reactivité: Humain, Porc

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD147 est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: MEM-M6-2

Détail du produit anti-CD147 anticorps

Fonction: Anti-Hu CD147 Purified

Specificité: The antibody MEM-M6/2 recognizes extracellular Ig domain D1 of CD147 (Neurothelin), a 50-60 kDa type I transmembrane glycoprotein primarily expressed on all leukocytes, red blood cells, platelets and endothelial cells, it is not expressed by resting lymphocytes. The antibody MEM-M6/1 is a high-affinity antibody capable of binding to unstimulated peripheral blood T cells.

Réactivité croisée (Details): Human, Porcine

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: A soluble recombinant form of CD147, CD147Rg, which consists of the cDNA coding for the entire extracellular region of CD147 fused to the DNA coding for the hinge region, CH2 and CH3 domain of human IgG1.

Isotype: IgG2b

Information d'application

Indications d'application: Flow cytometry: Recommended dilution: 4 μg/mL.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, positive tissue: testis.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-CD147 (ABIN93979)

Plánka, Necas, Srnec, Rauser, Starý, Jancár, Amler, Filová, Hlucilová, Kren, Gál: "Use of allogenic stem cells for the prevention of bone bridge formation in miniature pigs." dans: Physiological research / Academia Scientiarum Bohemoslovaca, Vol. 58, Issue 6, pp. 885-93, (2010) (PubMed).

Koch, Staffler, Hüttinger, Hilgert, Prager, Cerný, Steinlein, Majdic, Horejsí, Stockinger: "T cell activation-associated epitopes of CD147 in regulation of the T cell response, and their definition by antibody affinity and antigen density." dans: International immunology, Vol. 11, Issue 5, pp. 777-86, (1999) (PubMed).

Détails sur CD147

Antigène: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Autre désignation: CD147

Sujet: Basigin (Ok blood group),CD147 (basigin, neurothelin, OX-47, 5A11, CE9, M6) also known as EMMPRIN (extracellular matrix metalloproteinase inducer) or TCSF (tumour cell-derived collagenase-stimulatory factor) is an ubiquitously expressed cell surface protein with multiple glycosylated forms. The highest level of CD147 expression is on metabolically active cells, such as lymphoblasts, inflammatory cells, brown adipocytes and malignant tumour cells. CD147 has multiple functions, including facilitating of cell surface expression of monocarboxylate transporter proteins and extracellular matrix metalloproteinases, regulation of integrin functions, it plays roles in cell development and activation, fetal development or retinal function.,Neurothelin, Basigin, LAM6, CSF, 5F7, TCSF, BSG, TCSF, Emprin, OK blood group

ID gène: 682

UniProt: P35613

Pathways: S100 Proteins