CD16 anticorps

N° du produit ABIN93988

Cet anticorps Souris Monoclonal détecte spécifiquement CD16 dans FACS et IP. Il présente une réactivité envers Humain et Non-Human Primate et a été mentionné dans 4+ publications.

Aperçu rapide pour CD16 anticorps (ABIN93988)

Antigène: CD16

Reactivité: Humain, Non-Human Primate

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD16 est non-conjugé

Application: Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: LNK16

Détail du produit anti-CD16 anticorps

Fonction: Anti-Hu CD16 Purified

Specificité: The antibody LNK16 reacts with an extracellular epitope of CD16, a low affinity receptor for aggregated IgG (FcgammaRIII antigen). CD16 exists in two different isoforms: CD16a (FcgammaRIIIA, 50-65 kDa, expressed on NK-cells, monocytes and macrophages) and CD16b (FcgammaRIIIB, 48 kDa, mainly expressed on neutrophils).

Réactivité croisée (Details): Human, Non-Human Primates

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Normal human peripheral blood granulocytes

Isotype: IgG1

Information d'application

Indications d'application: Flow cytometry: Recommended dilution: 3-12 μg/mL.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-CD16 (ABIN93988)

Hovden, Karlsen, Jonsson, Aarstad, Appel: "Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses." dans: BMC immunology, Vol. 12, pp. 2, (2011) (PubMed).

Boyle: "Human macrophages kill human mesangial cells by Fas-L-induced apoptosis when triggered by antibody via CD16." dans: Clinical and experimental immunology, Vol. 137, Issue 3, pp. 529-37, (2004) (PubMed).

Mathison, Befus, Davison, Woodman: "Modulation of neutrophil function by the tripeptide feG." dans: BMC immunology, Vol. 4, pp. 3, (2003) (PubMed).

Tamm, Schmidt: "The binding epitopes of human CD16 (Fc gamma RIII) monoclonal antibodies. Implications for ligand binding." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 4, pp. 1576-81, (1996) (PubMed).

Détails sur CD16

Antigène: CD16

Autre désignation: CD16

Sujet: CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgammaRIII is expressed in two forms –, FcgammaRIII-A and -B. FcgammaRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-beta subunit. Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE. FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.,FcgammaRIII, IGFR3, FCRIII

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process