TERT anticorps (AA 597-611)

N° du produit ABIN940568

L’anticorps Chèvre Polyclonal anti-TERT a été validé pour ELISA, FACS et IF. Il convient pour détecter TERT dans des échantillons de Humain.

Aperçu rapide pour TERT anticorps (AA 597-611) (ABIN940568)

Antigène: TERT (Telomerase Reverse Transcriptase (TERT))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp TERT est non-conjugé

Application: ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Classe de qualité: Verified

Détail du produit anti-TERT anticorps

Épitope: AA 597-611

Fonction: TERT (aa597-611)

Séquence: QLRELSEAEV RQHRE

Specificité: This antibody is expected to recognize both reported isoforms (NP_937983.2, (NP_001180305.1).

Réactivité croisée: Humain

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-QLRELSEAEVRQHRE, from the internal region of the protein sequence according to NP_937983.2, NP_001180305.1.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Preliminary testing showed a band at approx 125 kDa in lysates of cell lines A549 and CaCo-2 after 1 μg/mL antibody staining (calculated MW of 127 kDa according to NP_937983.2). Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:32000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur TERT

Antigène: TERT (Telomerase Reverse Transcriptase (TERT))

Autre désignation: TERT

Sujet: TERT, telomerase reverse transcriptase, EST2, TCS1, TP2, TRT, hEST2, telomerase catalytic subunit, telomerase-associated protein 2

ID gène: 7015

NCBI Accession: NP_937983, NP_001180305

Pathways: Telomere Maintenance