CD44 anticorps

N° du produit ABIN94121

L’anticorps Souris Monoclonal anti-CD44 a été validé pour FACS, WB, IHC (p) et IP. Il convient pour détecter CD44 dans des échantillons de Humain, Chien et Porc. Il y a 2+ publications disponibles.

Aperçu rapide pour CD44 anticorps (ABIN94121)

Antigène: CD44

Reactivité: Humain, Chien, Porc

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD44 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Clone: MEM-263

Détail du produit anti-CD44 anticorps

Fonction: Anti-Hu CD44 Purified

Specificité: The antibody MEM-263 reacts with extracellular (N-terminal) domain of standard CD44 (Phagocyte glycoprotein 1), a 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.), it is negative on platelets and hepatocytes.

Réactivité croisée (Details): Human, Porcine, Canine (Dog)

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: COS-7 cells (African Green Monkey).

Isotype: IgG1

Information d'application

Indications d'application: Flow cytometry: Recommended dilution: 4 μg/mL.
Western blotting: Recommended dilution: 2 μg/mL, 60 min on vertical incubator, positive control: Kg-1a human acute leukemia cell lysate, JURKAT human leukemia T-cell lysate, non-reducing conditions, 6 % separating gel.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, positive tissue: uterus, myometrium.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-CD44 (ABIN94121)

Elashry, Gaertner, Klymiuk, Eldaey, Wenisch, Arnhold: "Characterisation of stemness and multipotency of ovine muscle-derived stem cells from various muscle sources." dans: Journal of anatomy, Vol. 239, Issue 2, pp. 336-350, (2021) (PubMed).

Klein, Weisshardt, Kleff, Jastrow, Jakob, Ergün: "Vascular wall-resident CD44+ multipotent stem cells give rise to pericytes and smooth muscle cells and contribute to new vessel maturation." dans: PLoS ONE, Vol. 6, Issue 5, pp. e20540, (2011) (PubMed).

Détails sur CD44

Antigène: CD44

Autre désignation: CD44

Sujet: CD44 Molecule,CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing, it can thus serve e.g. as a modulator of macrophage recruitment in response to pathogen. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumour growth and metastasis, antiapoptosis and directional motility of cancer cells.,PGP-I, HUTCH-I, ECMR-III, Hermes antigen, Hyaluronate receptor, Heparan sulfate proteoglycan, Epican, MC56, MIC4, INLU, LHR

ID gène: 960

UniProt: P16070

Pathways: Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling