Transferrin Receptor anticorps

N° du produit ABIN94226

L’anticorps Souris Monoclonal anti-Transferrin Receptor a été validé pour FACS, WB et IP. Il convient pour détecter Transferrin Receptor dans des échantillons de Humain. Il y a 1 publication disponible.

Aperçu rapide pour Transferrin Receptor anticorps (ABIN94226)

Antigène: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Transferrin Receptor est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP)

Clone: MEM-189

Détail du produit anti-Transferrin Receptor anticorps

Fonction: Anti-Hu CD71 Purified

Specificité: The antibody MEM-189 reacts with an extracellular epitope on CD71 antigen (transferrin receptor), a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and lymphocytes, macrophages and erythroid precursors, it is lost on resting blood leukocytes. The antibody MEM-189 does not block binding of transferrin to the receptor.

Réactivité croisée (Details): Human

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: KG1 human acute myelogenous leukaemia cell line

Isotype: IgG1

Information d'application

Indications d'application: Flow cytometry: Recommended dilution: 2 μg/mL.
Western blotting: Non-reducing conditions.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-Transferrin Receptor (ABIN94226)

Schatzlmaier, Supper, Göschl, Zwirzitz, Eckerstorfer, Ellmeier, Huppa, Stockinger: "Rapid multiplex analysis of lipid raft components with single-cell resolution." dans: Science signaling, Vol. 8, Issue 395, pp. rs11, (2015) (PubMed).

Détails sur Transferrin Receptor

Antigène: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Autre désignation: CD71

Sujet: Transferrin receptor,CD71 (transferrin receptor) is a type II transmembrane glycoprotein expressed as homodimer in erythroid blood cell line and in activated leukocytes. Upon binding of holotransferrin (complex of transferrin and iron ions), CD71 is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with CD71 and recycles to the cell surface, where it is released upon exposure to normal pH . CD71 is also involved in uptake of non-transferrin bound iron.,TfR1, TfR, TR, Trfr, T9, p90, TFRC

ID gène: 7037

UniProt: P02786

Pathways: Transition Metal Ion Homeostasis