Cytokeratin 18 anticorps

N° du produit ABIN94279

Cet anticorps Souris Monoclonal détecte spécifiquement Cytokeratin 18 dans WB, ELISA, IHC (p), IP, ICC et ICFC. Il présente une réactivité envers Mammifères et a été mentionné dans 4+ publications.

Aperçu rapide pour Cytokeratin 18 anticorps (ABIN94279)

Antigène: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Reactivité: Mammifères

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cytokeratin 18 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC), Intracellular Flow Cytometry (ICFC)

Clone: C-04

Détail du produit anti-Cytokeratin 18 anticorps

Fonction: Anti-Cytokeratin 18 Purified

Specificité: The antibody C-04 reacts with cytokeratin 18 (45 kDa intracellular antigen), a member of intermediate filaments subfamily represented in epithelial tissues.

Réactivité croisée (Details): Mammalian

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Cytoskeleton preparation of epidermal carcinoma cell line A431.

Isotype: IgG1

Information d'application

Indications d'application: Western blotting: Recommended dilution: 1-2 μg/mL.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, positive control: colon, kidney.
ELISA: The antibody C-04 has been tested as the capture antibody in a sandwich ELISA for analysis of cytokeratin 18 in combination with the antibody DA-7 (cat. no. 1B-110-C100) and with the antibody DC-10 (cat. no. 1B-107-C100).
Flow cytometry: Recommended dilution: 1-4 μg/mL. Intracellular staining.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-Cytokeratin 18 (ABIN94279)

Bártek, Vojt?sek, Stasková, Bártková, Kerekés, Rejthar, Kovarík: "A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology." dans: The Journal of pathology, Vol. 164, Issue 3, pp. 215-24, (1991) (PubMed).

Vojt?sek, Stasková, Nenutil, Lauerová, Kovarík, Rejthar, Bártková, Bártek: "Monoclonal antibodies recognizing different epitopes of cytokeratin No.18." dans: Folia biologica, Vol. 35, Issue 6, pp. 373-82, (1990) (PubMed).

Kovarík, Rejthar, Lauerová, Vojt?sek, Bártková: "Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread." dans: International journal of cancer. Supplement = Journal international du cancer. Supplement, Vol. 3, pp. 50-5, (1989) (PubMed).

Lauerová, Kovarik, Bártek, Rejthar, Vojt?sek: "Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule." dans: Hybridoma, Vol. 7, Issue 5, pp. 495-504, (1989) (PubMed).

Détails sur Cytokeratin 18

Antigène: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Autre désignation: Cytokeratin 18

Sujet: Keratin 18,Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Cytokeratin 18 belongs to type I family (acidic cytokeratins).,K18, CK18, CYK18, KRT18

ID gène: 3875

UniProt: P05783

Pathways: Apoptose, Caspase Cascade in Apoptosis