Cytokeratin 18 anticorps (Biotin)

N° du produit ABIN94283

L’anticorps Souris Monoclonal anti-Cytokeratin 18 a été validé pour WB, FACS, ELISA, IHC (p), IP et ICC. Il convient pour détecter Cytokeratin 18 dans des échantillons de Humain. Il y a 3+ publications disponibles.

Aperçu rapide pour Cytokeratin 18 anticorps (Biotin) (ABIN94283)

Antigène: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cytokeratin 18 est conjugé à/à la Biotin

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: DC-10

Détail du produit anti-Cytokeratin 18 anticorps (Biotin)

Fonction: Anti-Cytokeratin 18 Biotin

Specificité: The mouse monoclonal antibody DC-10 reacts with cytokeratin 18 (45 kDa). Cytokeratins are members of intermediate filaments subfamily of intracellular proteins, represented in epithelial tissues.

Aucune reactivité croisée: Boeuf (Vache), Chien, Hamster, Souris, Porc, Rat, Mouton

Réactivité croisée (Details): Human

Purification: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.

Immunogène: Human breast carcinoma cell line PMC-42.

Isotype: IgG1

Information d'application

Indications d'application: Flow cytometry: Recommended dilution: 2-6 μg/mL. Intracellular staining.
Western blotting: Recommended dilution: 1-2 μg/mL.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.
Avoid prolonged exposure to light.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-Cytokeratin 18 (Biotin) (ABIN94283)

Vojt?sek, Stasková, Nenutil, Lauerová, Kovarík, Rejthar, Bártková, Bártek: "Monoclonal antibodies recognizing different epitopes of cytokeratin No.18." dans: Folia biologica, Vol. 35, Issue 6, pp. 373-82, (1990) (PubMed).

Kovarík, Rejthar, Lauerová, Vojt?sek, Bártková: "Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread." dans: International journal of cancer. Supplement = Journal international du cancer. Supplement, Vol. 3, pp. 50-5, (1989) (PubMed).

Lauerová, Kovarik, Bártek, Rejthar, Vojt?sek: "Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule." dans: Hybridoma, Vol. 7, Issue 5, pp. 495-504, (1989) (PubMed).

Détails sur Cytokeratin 18

Antigène: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Autre désignation: Cytokeratin 18

Sujet: Keratin 18,Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Cytokeratin 18 belongs to type I family (acidic cytokeratins).,K18, CK18, CYK18, KRT18

ID gène: 3875

UniProt: P05783

Pathways: Apoptose, Caspase Cascade in Apoptosis