Acrosin anticorps

N° du produit ABIN94455

Cet anticorps anti-Acrosin est un anticorps Souris Monoclonal détectant Acrosin dans WB, FACS et ICC. Adapté pour Porc. Ce Primary Antibody a été cité dans 7+ publications.

Aperçu rapide pour Acrosin anticorps (ABIN94455)

Antigène: Acrosin (ACR)

Reactivité: Porc

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Acrosin est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: ACR-2

Détail du produit anti-Acrosin anticorps

Fonction: Anti-Por Acrosin Purified

Specificité: The antibody ACR-2 reacts with various forms of porcine acrosin (55, 53, 45 and 35 kDa), a typical serine proteinase with trypsin-like specificity. Acrosin is stored in the acrosome of undamaged spermatozoa.

Aucune reactivité croisée: Boeuf (Vache), Chien, Humain

Réactivité croisée (Details): Porcine

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Acid extracts of boar spermatozoa were subjected to hydrophobic chromatography and the pooled fraction with reactivity to N-alpha benzoylarginine-4-nitroanilide was used for immunization.

Isotype: IgG1

Information d'application

Indications d'application: Flow cytometry: Recommended dilution: 1-12 μg/mL. Intracellular staining.
Immunocytochemistry: Membrane permeabilization (acetone) is essential.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-Acrosin (ABIN94455)

Ded, Dostalova, Zatecka, Dorosh, Komrskova, Peknicova: "Fluorescent analysis of boar sperm capacitation process in vitro." dans: Reproductive biology and endocrinology : RB&E, Vol. 17, Issue 1, pp. 109, (2020) (PubMed).

Ded, Dostalova, Dorosh, Dvorakova-Hortova, Peknicova: "Effect of estrogens on boar sperm capacitation in vitro." dans: Reproductive biology and endocrinology : RB&E, Vol. 8, pp. 87, (2010) (PubMed).

Peknicova, Capkova, Geussova, Ivanova, Mollova: "Monoclonal antibodies to intra-acrosomal proteins inhibit gamete binding in vitro." dans: Theriogenology, Vol. 56, Issue 2, pp. 211-23, (2001) (PubMed).

Moos, Peknicova, Tesarik: "Protein-protein interactions controlling acrosin release and solubilization during the boar sperm acrosome reaction." dans: Biology of reproduction, Vol. 49, Issue 2, pp. 408-15, (1993) (PubMed).

Moos, Peknicova, Tesarik: "Relationship between molecular conversions of acrosin and the progression of exocytosis in the calcium ionophore-induced acrosome reaction." dans: Biochimica et biophysica acta, Vol. 1176, Issue 3, pp. 199-207, (1993) (PubMed).

Moos, Tesarik, Leca, Peknicova: "Mechanism of maturation and nature of carbohydrate chains of boar sperm acrosin." dans: FEBS letters, Vol. 294, Issue 1-2, pp. 27-30, (1992) (PubMed).

Peknicová, Moos: "Monoclonal antibodies against boar acrosomal antigens labelling undamaged acrosomes of spermatozoa in immunofluorescence test." dans: Andrologia, Vol. 22, Issue 5, pp. 427-35, (1991) (PubMed).

Détails sur Acrosin

Antigène: Acrosin (ACR)

Autre désignation: Acrosin

Sujet: Acrosin,Acrosin is a serine proteinase expressed in the acrosome of mature spermatozoa. This enzyme facilitates penetration of the sperm through the zona pellucida of the ovum.,ACR, FBP53

ID gène: 397098

UniProt: P08001

Pathways: cAMP Metabolic Process