Acetylcholinesterase anticorps (Biotin)

N° du produit ABIN94540

N° du produit (Fournisseur): 200-106-232-0100

Cet anticorps anti-Acetylcholinesterase est un anticorps Chèvre Polyclonal détectant Acetylcholinesterase dans WB, ELISA et IP. Adapté pour Boeuf (Vache).

Aperçu rapide pour Acetylcholinesterase anticorps (Biotin) (ABIN94540)

Antigène: Acetylcholinesterase (AChE)

Reactivité: Boeuf (Vache)

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Acetylcholinesterase est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Détail du produit anti-Acetylcholinesterase anticorps (Biotin)

N° du produit (Fournisseur): 200-106-232-0100

Fournisseur: Rockland

Fonction: Acetyl Cholinesterase Antibody Biotin Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Acetyl Cholinesterase [Bovine Erythrocytes].

Attributs du produit: Synonyms: goat anti-Acetyl Cholinesterase Antibody, biotin conjugated goat anti-Acetyl Cholinesterase Antibody, Acetylcholinesterase, AChE

Purification: Acetyl Cholinesterase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogène:

Immunogen: Acetyl Cholinesterase [Bovine Erythrocytes]

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Anti-Acetyl Cholinesterase Biotin Conjugated Antibody has been tested by western blot and is suitable to be assayed against 1.0 μg of Acetyl Cholinesterase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:2,000 is suggested for this product.

Western Blot Dilution: 1:500 - 1:2,500

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:2,000 - 1:10,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Détails sur Acetylcholinesterase

Antigène: Acetylcholinesterase (AChE)

Autre désignation: ACHE

Sujet: Background: Acetyl Cholinesterase is abundantly expressed in erythrocytes and is part of the type-B carboxylesterase/lipase family. Anti-Acetyl Cholinesterase cancels signal transduction at the neuromuscular junction via hydrolysis of acetylcholine as it is released into the synaptic cleft by the motor neurons. Anti-Acetyl Cholinesterase antibody is ideal for investigators involved in Neuroscience and Neurotransmitter research.

ID gène: 540446

NCBI Accession: NP_001069688

UniProt: P23795

Pathways: Skeletal Muscle Fiber Development