Amylase, alpha anticorps

N° du produit ABIN95004

N° du produit (Fournisseur): 200-401-045

Cet anticorps Lapin Polyclonal détecte spécifiquement Amylase, alpha dans WB, ELISA et IP. Il présente une réactivité envers Bacillus amyloliquefaciens.

Aperçu rapide pour Amylase, alpha anticorps (ABIN95004)

Antigène: Amylase, alpha (AMY)

Reactivité: Bacillus amyloliquefaciens

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Amylase, alpha est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Détail du produit anti-Amylase, alpha anticorps

N° du produit (Fournisseur): 200-401-045

Fournisseur: Rockland

Fonction: Alpha Amylase Antibody

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified a-Amylase [Bacillus amyloliquefaciens].

Attributs du produit: Synonyms: rabbit anti-Alpha Amylase Antibody, Alpha-amylase, 1,4-alpha-D-glucan glucanohydrolase

Purification: Alpha Amylase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Stérilité: Sterile filtered

Immunogène:

Immunogen: a-Amylase [Bacillus amyloliquefaciens]

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Anti-Alpha Amylase has been tested by ELISA and western blot and is assayed against 1.0 μg of a-Amylase [Bacillus amyloliquefaciens] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store Alpha Amylase Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Anti-Alpha Amylase Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur Amylase, alpha

Antigène: Amylase, alpha (AMY)

Autre désignation: Alpha-amylase

Sujet: Background: Alpha Amylase is a protein enzyme that preforms endohydrolysis of alpha bonds in large alpha linked polysaccharides, such as starch and glycogen, yielding glucose and maltose. Alpha Amylase applications include ethanol production and reduction of starch or glycogen molecules. Anti-Alpha Amylase Antibody is useful for researchers interested in Metabolism, Cancer, Cell Signaling, and Signal Transduction research.

ID gène: 7849308

NCBI Accession: WP_013352208

UniProt: P00692