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His Tag anticorps

Cet anticorps anti-His Tag est un anticorps Poulet Polyclonal détectant His Tag dans WB. Adapté pour .
N° du produit ABIN950190

Aperçu rapide pour His Tag anticorps (ABIN950190)

Antigène

Voir toutes His Tag Anticorps
His Tag

Reactivité

Veuillez nous consulter SVP

Hôte

  • 111
  • 66
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  • 1
Poulet

Clonalité

  • 126
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Polyclonal

Conjugué

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  • 1
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  • 1
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Cet anticorp His Tag est non-conjugé

Application

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Western Blotting (WB)
  • Séquence

    HHHHHH

    Purification

    Purified

    Immunogène

    6X-Histidine peptide conjugated to Keyhole Limpet Hemocyanin protein and emulsified in Freund’s adjuvants. After multiple injections, eggs were collected from the hens, IgY fractions were prepared from the yolks, and then affinity-purified antibodies were prepared using 6X-Histidine peptide conjugated to an agarose matrix. To make up the final mixture, the IgY fraction was spiked with affinity purified antibody, mixed with Glycerol and Thimerosal, and filter-sterilized.

    Isotype

    Ig Fraction
  • Indications d'application

    Optimal working dilution should be determined by the investigator.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    10 mg/mL (based on absorbance at 280 nm)

    Buffer

    Sodium Phosphate-Buffered (10 mM) isotonic (0.9 % , w/v) Saline (PBS, pH 7.2) (50 % by volume) with 50 % Glycerol as an anti-freezing agent and 0.01 % Thimerosal as preservative.

    Agent conservateur

    Thimerosal (Merthiolate)

    Précaution d'utilisation

    This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Antigène

    His Tag

    Autre désignation

    6xHistidine Epitope Tag

    Classe de substances

    Tag

    Sujet

    In the last couple of years many peptide sequences/epitopes for the purification of recombinant proteins have been established. These so-called Synonyms: 6xHis-Tag, HHHHHH Tag, HIS6 Tag, His Tag
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