6X-Histidine peptide conjugated to Keyhole Limpet Hemocyanin protein and emulsified in Freund’s adjuvants. After multiple injections, eggs were collected from the hens, IgY fractions were prepared from the yolks, and then affinity-purified antibodies were prepared using 6X-Histidine peptide conjugated to an agarose matrix. To make up the final mixture, the IgY fraction was spiked with affinity purified antibody, mixed with Glycerol and Thimerosal, and filter-sterilized.
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Format
Liquid
Concentration
10 mg/mL (based on absorbance at 280 nm)
Buffer
Sodium Phosphate-Buffered (10 mM) isotonic (0.9 % , w/v) Saline (PBS, pH 7.2) (50 % by volume) with 50 % Glycerol as an anti-freezing agent and 0.01 % Thimerosal as preservative.
Agent conservateur
Thimerosal (Merthiolate)
Précaution d'utilisation
This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Antigène
His Tag
Autre désignation
6xHistidine Epitope Tag
Classe de substances
Tag
Sujet
In the last couple of years many peptide sequences/epitopes for the purification of recombinant proteins have been established. These so-called Synonyms: 6xHis-Tag, HHHHHH Tag, HIS6 Tag, His Tag