APP anticorps

N° du produit ABIN95037

N° du produit (Fournisseur): 600-401-253

Cet validé indépendamment anticorps Lapin Polyclonal détecte spécifiquement APP dans WB, ELISA, IHC, IF et FM. Il présente une réactivité envers Humain et Souris et a été mentionné dans 1 publication.

Aperçu rapide pour APP anticorps (ABIN95037)

Antigène: APP (Amyloid beta (A4) Precursor Protein (APP))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp APP est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Détail du produit anti-APP anticorps

N° du produit (Fournisseur): 600-401-253

Fournisseur: Rockland

Fonction: Beta Amyloid Antibody

Réactivité croisée (Details): This affinity purified antibody is directed against extracellular region of beta amyloid and is useful in determining its presence in various assays.

Attributs du produit: Synonyms: rabbit anti-Beta Amyloid Antibody, ß-amyloid, Amyloid beta A4 protein, Alzheimer disease amyloid protein, Beta amyloid, A-beta, ABPP, APPI, Beta-amyloid precursor protein

Purification: affinity purified antibody

Stérilité: Sterile filtered

Immunogène:

Immunogen: This antibody was affinity purified from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to an extracellular region of human beta amyloid conjugated to KLH using maleimide.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:50-1:200

Application Note: Affinity purified anti-beta amyloid has been tested by ELISA, IHC, WB, and IF. A 45.8 kDa band is detected in western blot using whole tissue extracts and lysates from mouse and human.  In general, we recommend the use of 4 % PFA or 10 % formalin for fixation of tissues with IHC-paraffin or IHC-frozen application of this antibody.

Western Blot Dilution: 1:1,000-1:5000

ELISA Dilution: 1:10,000 - 1:30,000

IF Microscopy Dilution: 1:50-1:200

Other: User Optimized

Restrictions: For Research Use only

Independent Validation (UP)

Validation #104082 (Unfolding Profile)

by: NanoTemper Technologies

No.: #104082

Date: 23.07.2019

Antigène: ABETA

Application validée: Unfolding Profile

Contrôle positif: ABIN1043866

Conclusion:

Passed. ABIN1043866 showed T_i at 83.8°C and a clear unfolding profile with one unfolding event. This suggests that the antibody is properly folded and functional.

Validation Images

Unfolding profile of ABIN1043866. The fluorescence signal is plotted against temperature. The vertical line indicates the Ti at 83.8 °C.

Protocole

Full Protocol:

• Load the undiluted sample into Tycho capillary (NanoTemper Technologies, TY-C001).
• Run Tycho measurement.

Notes:

Tycho is designed to run quick and precise protein quality check experiments. Tycho uses intrinsic protein fluorescence to follow protein unfolding while running a fast thermal ramp, yielding results in 3min. A protein’s unfolding behavior is characterized by various parameters, most notably the inflection temperature (T_i). The T_i can be used to identify properly folded protein, to compare different batches, or to analyze the influence of storage/transport conditions on a protein. An absence of T_i would suggest that the protein is already unfolded and therefore most likely nonfunctional.

Independent Validation (IHC)

Validation #104424 (Immunohistochemistry)

by: Prof. Merighi, Laboratory of Neurobiology, Department of Veterinary Sciences, University of Turin

No.: #104424

Date: 12.05.2022

Antigène: beta Amyloid

Numéro du lot: 42581

Application validée: Immunohistochemistry

Contrôle positif:

Adult (24 months) mouse brain

3-month-old Taconic mouse Model 1349 brain fixed in 4% paraformaldehyde

Contrôle négative:

We incubated slices overnight with the blocking solution only and then processed them with the secondary antibody.

Conclusion:

The beta Amyloid antibody (Amyloid beta) (N-Term) ABIN95037 works in IHC-P, especially at higher concentrations (1:20 and 1:50), and without the use of any antigen retrieval treatment.

Validation Images

Staining of beta-amyloid positive cells in the adult mouse brain using ABIN95037 at 40x magnification.

Protocole

Anticorps primaire: ABIN95037

Anticorps secondaire: goat anti-rabbit AF488-conjugated antibody (Invitrogen by Thermo Fisher Scientific, A11034, lot 1971418)

Full Protocol:

• Perfuse mice with paraformaldehyde 4% in 0.1 M phosphate buffer pH 7.4 and post-fix in the same fixative for an additional 2 h at RT.
• Wash, dehydrate, and embed samples in paraffin wax.
• Wash several times with 0.01 M PBS.
• Cut intestines and brain with a microtome into 6µm sections and mount on glass slides.
• After paraffin removal, incubate sections for 1 h at RT in PBS containing 1% albumin from chicken egg white (Sigma, A5378) and 0.3% Triton-X-100 (BioRad, 161-0407, lot 00583) to block non-specific binding sites.
• Incubate sections with primary anti-beta amyloid (N-Term) (antibodies Online, ABIN95037, lot 42581) diluted 1:20, 1:50, 1:100, and 1:200 in 0.1 M PBS-BSA-PLL ON at RT.
• Wash sections 3x 5 min with 0.01 M PBS.
• Incubate sections with secondary goat anti-rabbit AF488-conjugated antibody (Invitrogen by Thermo Fisher Scientific, A11034, lot 1971418) diluted 1:500 in 0.1 M PBS for 1 h at RT.
• Wash sections 3x 5 min with 0.01M PBS.
• Mount specimens in Fluoroshield (Sigma, F6182, lot MKCB0153V).
• Acquire images with a fluorescence microscope and appropriate filter settings for AF488, e.g. Leica DM 6000B fluorescence microscope equipped with a digital camera at 40x magnification.

Notes:

Antigen retrieval treatment was also tested. In this case, sections were processed for microwave antigen retrieval for 10 min (95-100 °C) in 10 mM sodium citrate buffer (pH 6.0). After 20 min of spontaneous cooling, sections were washed twice for 5 min with distilled water and for 5 min with PBS.

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-APP (ABIN95037)

Frenkel, Balass, Solomon: "N-terminal EFRH sequence of Alzheimer's beta-amyloid peptide represents the epitope of its anti-aggregating antibodies." dans: Journal of neuroimmunology, Vol. 88, Issue 1-2, pp. 85-90, (1998) (PubMed).

Détails sur APP

Antigène: APP (Amyloid beta (A4) Precursor Protein (APP))

Autre désignation: APP

Sujet: Background: Beta amyloid, often abbreviated as A-beta, is a protein that builds up in the brains of persons with Alzheimer's disease, collecting in clumps called plaques or senile plaques. While some researchers question whether beta amyloid is the cause of the dementia, most agree that it is involved in the disruption of thinking that is a hallmark of the disease. In some cases of familial Alzheimer's disease, mutations in genes for the proteins called the presenilins lead to increased production of amyloid. Researchers have been looking at how presenilin-1 in particular contributes to the excess buildup of beta amyloid. Presenilin-1 apparently acts to increase the activity of gamma-secretase, an enzyme that changes a normal protein (amyloid precursor protein or APP) into beta amyloid itself. Furthermore, presenilin-1 might be gamma-secretase. Anti-Beta Amyloid Antibody is useful for researchers interested in TLR signaling and Alzheimer's research.

ID gène: 351

NCBI Accession: NP_000475

UniProt: P05067

Pathways: Caspase Cascade in Apoptosis, EGFR Signaling Pathway, Transition Metal Ion Homeostasis, Skeletal Muscle Fiber Development, Toll-Like Receptors Cascades, Feeding Behaviour