Transferrin Receptor anticorps (PE)

N° du produit ABIN951380

L’anticorps Rat Monoclonal anti-Transferrin Receptor a été validé pour FACS, IP et IHC (fro). Il convient pour détecter Transferrin Receptor dans des échantillons de Souris.

Aperçu rapide pour Transferrin Receptor anticorps (PE) (ABIN951380)

Antigène: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp Transferrin Receptor est conjugé à/à la PE

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: R17217-1-4

Détail du produit anti-Transferrin Receptor anticorps (PE)

Specificité: This antibody recognizes the Transferrin receptor protein on the surface of most dividing cells.

Attributs du produit: Synonyms: TfR1, p90, Transferrin receptor protein 1

Purification: Protein G Chromatography

Isotype: IgG2a

Information d'application

Indications d'application: Flow Cytometry: 1g to stain 1.0 x 10^6 cells. Immunoprecipitation. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.1 mg/mL (OD280nm)

Buffer: 0.01 M PBS pH 7.2 containing 2.0 mM EDTA, 1.0 % BSA and 0.09 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur Transferrin Receptor

Antigène: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Autre désignation: CD71 / TFRC

Sujet: Cellular uptake of iron occurs via receptor mediated endocytosis of ligand occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). Useful in studies of dividing haematopoietic and tumour cell populations, and metabolic activity. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C terminal binding site. The antigen is present on most dividing cells, including normally cycling in vivo hematopoietic progenitor cells, mitogenically stimulated cells in vitro, some primary tumor cells and most proliferating cells in vitro. The transferrin receptor has been structurally characterized as a sulfide bound dimer of identical glycoprotein subunits of 95 kDa. The transferrin receptor is not present on resting blood lymphocytes. On PBL, the receptor appears after activation. The expression of transferrin receptor is coordinately regulated with cell growth. Present on T and B cell lines. The soluble (or serum) transferrin receptor (sTfR) is a circulating truncated form of the membrane receptor protein, it is an 85 kDa glycoprotein forming in serum a 320 kDa complex with diferric transferrin. The most important clinical use of the sTfR determination is in the differential diagnosis between iron deficiency anaemia and the anaemia of chronic disease.Synonyms: TfR1, Transferrin receptor protein 1, p90

ID gène: 22042

UniProt: Q62351

Pathways: Transition Metal Ion Homeostasis