DYKDDDDK Tag anticorps

N° du produit ABIN952003

Cet anticorps Poulet Polyclonal détecte spécifiquement DYKDDDDK Tag dans WB, IP, IF, EIA et IHC (fro).

Aperçu rapide pour DYKDDDDK Tag anticorps (ABIN952003)

Antigène: DYKDDDDK Tag

Hôte: Poulet

Clonalité: Polyclonal

Conjugué: Cet anticorp DYKDDDDK Tag est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-DYKDDDDK Tag anticorps

Séquence: DYKDDDDK

Purification: Affinity Chromatography.

Immunogène: Synthetic peptide containing the DYKDDDDK epitope conjugated to Keyhole Limpet Hemocyanin (KLH). Production: The first injection included complete Freund’s adjuvant, subsequent injections included incomplete Freund’s adjuvant. Eggs were collected after the fourth injection, and antibodies were purified from the yolks using a proprietary method that yields a purity of >90%. Antibodies were then affinity-purified over a peptide column and dialyzed against Phosphate-Buffered isotonic Saline. Sodium azide was added, the protein concentration was adjusted and the antibodies were filter-sterilized (0.2 mym).

Isotype: Ig Fraction

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL (based on absorbance at 280 nm)

Buffer: PBS, pH 7.2 containing 0.02 % (w/v) Sodium Azide as an anti-microbial agent.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted in the dark at 2-8 °C.

Détails sur DYKDDDDK Tag

Antigène: DYKDDDDK Tag

Autre désignation: DYKDDDDK Epitope Tag

Classe de substances: Tag

Sujet: Sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.Synonyms: D-tag, ECS Epitope Tag, ECS-tag, FLAG Epitope Tag, FLAG-tag