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ENO2/NSE anticorps

Cet anticorps anti-ENO2/NSE est un anticorps Poulet Polyclonal détectant ENO2/NSE dans WB, IF et IHC (p). Adapté pour Humain, Souris et Rat.
N° du produit ABIN953676

Aperçu rapide pour ENO2/NSE anticorps (ABIN953676)

Antigène

Voir toutes ENO2/NSE (ENO2) Anticorps
ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Reactivité

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Humain, Souris, Rat

Hôte

  • 112
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Poulet

Clonalité

  • 117
  • 104
Polyclonal

Conjugué

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Cet anticorp ENO2/NSE est non-conjugé

Application

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Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • Specificité

    Recognizes Neuron-Specific Enolase-2 (NSE).

    Purification

    Affinity Chromatography using a peptide column.

    Immunogène

    Chickens were immunized with two synthetic peptide KLH conjugates corresponding to different regions of the NSE-2 gene product, but are shared between the Human (NP_001966, NCBI) and Rat (AAA41119) sequences. Production: After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.2 mg/ml. Finally, equal volumes of each of these affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

    Isotype

    Ig Fraction
  • Indications d'application

    Optimal working dilution should be determined by the investigator.

    Restrictions

    For Research Use only
  • Buffer

    10 mM PBS, pH 7.2 containing 0.02 % Sodium Azide as preservative.

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Stock

    4 °C

    Stockage commentaire

    Store the antibody undiluted in the dark at 2-8 °C.
  • Antigène

    ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

    Autre désignation

    Neuron Specific Enolase

    Sujet

    Human NSE-2 (EC 4.2.1.11) is a 47,138 dalton protein (434 amino acids) expressed in neurons of the peripheral nervous system (PNS) and central nervous system (CNS). NSE-2 catalyzes the conversion of 2-phospho-D-glycerate into phosphoenol pyruvate, and is an essential enzyme in energy metabolism in nervous tissues.Synonyms: 2-phospho-D-glycerate hydro-lyase, ENO2, Enolase 2, Gamma-enolase, NSE, Neural enolase

    ID gène

    2026

    NCBI Accession

    NP_001966
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