CPY anticorps

N° du produit ABIN96043

N° du produit (Fournisseur): 100-401-135

Cet anticorps Lapin Polyclonal détecte spécifiquement CPY dans WB et ELISA. Il présente une réactivité envers Saccharomyces cerevisiae et a été mentionné dans 3+ publications.

Aperçu rapide pour CPY anticorps (ABIN96043)

Antigène: CPY (Carboxypeptidase Y (CPY))

Reactivité: Saccharomyces cerevisiae

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CPY est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-CPY anticorps

N° du produit (Fournisseur): 100-401-135

Fournisseur: Rockland

Fonction: Carboxypeptidase Y Antibody

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Carboxypeptidase Y [Baker's Yeast].

Attributs du produit: Synonyms: rabbit anti-Carboxypeptidase Y antibody, Carboxypeptidase YSCY antibody, CPY1 antibody, LBC1 antibody, PRC1 antibody, Vacuolar carboxypeptidase Y antibody, YMR297W antibody

Purification: This product was prepared from monospecific antiserum by a delipidation and defibrination.

Immunogène:

Immunogen: Carboxypeptidase Y [Baker's Yeast]

Immunogen Type: Native Protein

Information d'application

Indications d'application:

Application Note: Anti-Carboxypeptidase Y has been tested by western blot and is suitable to be assayed against 1.0 μg of Carboxypeptidase Y [Baker's Yeast] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:5,000 - 1:25,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 90 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-CPY (ABIN96043)

Martinez Benitez, Stolz, Becher, Wolf: "Mnl2, a novel component of the ER associated protein degradation pathway." dans: Biochemical and biophysical research communications, (2011) (PubMed).

Ohashi, Munro: "Membrane delivery to the yeast autophagosome from the Golgi-endosomal system." dans: Molecular biology of the cell, Vol. 21, Issue 22, pp. 3998-4008, (2011) (PubMed).

von Numers, Survila, Aalto, Heino, Palva, Li: "Requirement of a Homolog of Glucosidase II {beta}-Subunit for EFR-Mediated Defense Signaling in Arabidopsis thaliana." dans: Molecular plant, (2010) (PubMed).

Détails sur CPY

Antigène: CPY (Carboxypeptidase Y (CPY))

Autre désignation: Carboxypeptidase Y

Sujet: Background: Carboxypeptidase Y is involved in degradation of small peptides. It digests preferentially peptides containing an aliphatic or hydrophobic residue in P1' position, as well as methionine, leucine or phenylalanine in P1 position of ester substrate. Carboxypeptidase that catalyzes the release of a C-terminal amino acid with broad specificity. It is inhibited by ZPCK.

ID gène: 855343

NCBI Accession: NP_014026

UniProt: P00729