alpha Tubulin anticorps (C-Term, Subunit alpha)

N° du produit ABIN962076

Cet anticorps anti-alpha Tubulin Monoclonal Rat (Clone YL1-2) (ABIN962076) détecte spécifiquement alpha Tubulin dans WB, ELISA, IHC, IHC (p), IP, IHC (fro) et RIA. L’anticorps est réactif avec des échantillons de Humain, Saccharomyces cerevisiae, Mammifères, Avian et Echinodermata.

Aperçu rapide pour alpha Tubulin anticorps (C-Term, Subunit alpha) (ABIN962076)

Antigène: alpha Tubulin (TUBA1)

Reactivité: Humain, Saccharomyces cerevisiae, Mammifères, Avian, Echinodermata

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp alpha Tubulin est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Radioimmunoassay (RIA)

Clone: YL1-2

Détail du produit anti-alpha Tubulin anticorps

Épitope: C-Term, Subunit alpha

Specificité: Recognizes the alpha subunit of tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin). The epitope recognized by this has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin). The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin. This product is routinely tested in ELISA on Tubulin.

Purification: Affinity purified

Immunogène: Yeast tubulin.

Type of Immunogen: Purified protein

Isotype: IgG2a

Information d'application

Indications d'application: Approved: ELISA (1:100 - 1:1000), IHC, IHC-Fr, IHC-P (5 μg/mL), IP, RIA, WB

Commentaires:

Target Species of Antibody: Yeast

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur alpha Tubulin

Antigène: alpha Tubulin (TUBA1)

Autre désignation: Alpha Tubulin

Pathways: Dynamique des Microtubules